AB189826

Anti-Pea3 antibody

Name: Anti-Pea3 antibody (ab189826) | Abcam
Brand: Abcam Limited
SKU: AB189826
Price: 430 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(11 Publications)

Rabbit Polyclonal Pea3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ETV4.

View Alternative Names

E1AF, PEA3, ETV4, ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Pea3 antibody (AB189826)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Pea3 antibody (AB189826)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab189826. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-Pea3 antibody (AB189826)

All lanes:

Western blot - Anti-Pea3 antibody (ab189826) at 1/500 dilution

Lane 1:

SW480 cell extract

Lane 2:

H460 cell extract

Lane 3:

SKOV3 cell extract

Lane 4:

BT474 cell extract

Lane 5:

Raji cell extract

Lane 6:

Mouse skeletal muscle cell extract

Lane 7:

Mouse heart cell extract

Predicted band size: 54 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ETV4. The exact immunogen used to generate this antibody is proprietary information.

P43268

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pea3 also called PEA3 or ETV4 functions as a transcription factor from the ETS family. It has a mass of approximately 55 kDa. This protein regulates gene expression by binding to specific DNA sequences. Pea3 is mainly expressed in the brain lung and liver tissues where it influences the transcription of a range of target genes. It plays an important role in mediating cellular responses to extracellular signals.

Biological function summary

Pea3 influences key processes like cell proliferation differentiation and migration. It often operates within larger protein complexes to execute these roles. Pea3 has been shown to regulate genes involved in development and oncogenic processes by interacting with co-factors in transcriptional complexes. By activating or repressing the transcription of specific genes Pea3 contributes to the dynamic regulation of cellular environments.

Pathways

Several intracellular signaling pathways involve Pea3 in integrating cellular responses to external stimuli. Notably Pea3 plays a role in the MAPK/ERK pathway where it works alongside proteins like ERK and other transcription factors to mediate cell growth and differentiation. Pea3 also participates in the PI3K/AKT pathway influencing cell survival and metabolism. In these pathways Pea3 acts as a bridge linking extracellular signals to transcriptional outputs.

Pea3 connects to cancer progression and metastasis. Overexpression of Pea3 is associated with various cancers including breast and lung cancer where it interacts with proteins like matrix metalloproteinases (MMPs) to promote metastasis. Pea3 also links to neurological disorders by influencing genes involved in brain development and function demonstrating its widespread significance in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator (PubMed : 19307308, PubMed : 31552090). May play a role in keratinocyte differentiation (PubMed : 31552090).. (Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator; the core-binding sequence is 5'-[AC]GGA[AT]GT-3'.

See full target information ETV4

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1595850 PubMed40469297

2025

Comprehensive analysis of the functional and immunological significance of ETV4 in pan-cancer and its validation in digestive tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Lingli Huang,Xujia Li,Silan Huang,Qi Jiang,Chang Jiang,Wenzhuo He,Yuchen Cai,Guifang Guo

Cell death & disease 15:738 PubMed39389944

2024

The pathogenic germline ETV4 P433L mutation identified in multiple primary lung cancer affect tumor stem-like property by Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Liu,Lingling Fang,Yalong Wang,Tao Fan,Liyu Wang,Chu Xiao,Ziqin Deng,Wenpeng Cai,Bo Zheng,Junfeng Qiu,Chunxiang Li,Jie He

World journal of gastrointestinal oncology 16:4014-4027 PubMed39350976

2024

Prognostic value of subfamily gene expression in cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Zhe Zhang,Hai-Zhang Ma

Journal of translational medicine 22:547 PubMed38849954

2024

An oncogenic enhancer promotes melanoma progression via regulating ETV4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Junyou Zhang,Qilin Wang,Sihan Qi,Yingying Duan,Zhaoshuo Liu,Jiaxin Liu,Ziyi Zhang,Chunyan Li

Clinical cancer research : an official journal of the American Association for Cancer Research 29:3438-3456 PubMed37406085

2023

Combined CDK4/6 and ERK1/2 Inhibition Enhances Antitumor Activity in NF1-Associated Plexiform Neurofibroma.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa C Flint,Dana K Mitchell,Steven P Angus,Abbi E Smith,Waylan Bessler,Li Jiang,Henry Mang,Xiaohong Li,Qingbo Lu,Brooke Rodriguez,George E Sandusky,Andi R Masters,Chi Zhang,Pengtao Dang,Jenna Koenig,Gary L Johnson,Weihua Shen,Jiangang Liu,Amit Aggarwal,Gregory P Donoho,Melinda D Willard,Shripad V Bhagwat,D Wade Clapp,Steven D Rhodes

Oncogene 42:434-448 PubMed36509998

2022

Targeting PEA3 transcription factors to mitigate small cell lung cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

David W Shia,WooSuk Choi,Preethi Vijayaraj,Valarie Vuong,Jenna M Sandlin,Michelle M Lu,Adam Aziz,Caliope Marin,Cody J Aros,Chandani Sen,Abdo Durra,Andrew J Lund,Arunima Purkayastha,Tammy M Rickabaugh,Thomas G Graeber,Brigitte N Gomperts

Cell death & disease 13:116 PubMed35121725

2022

HBx increases chromatin accessibility and ETV4 expression to regulate dishevelled-2 and promote HCC progression.

Applications

Unspecified application

Species

Unspecified reactive species

Chuqian Zheng,Min Liu,Yanping Ge,Yanyan Qian,Hong Fan

Experimental neurobiology 30:294-307 PubMed34483143

2021

Hyperoxygenation Treatment Reduces Beta-amyloid Deposition via MeCP2-dependent Upregulation of MMP-2 and MMP-9 in the Hippocampus of Tg-APP/PS1 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Juli Choi,Hyejin Kwon,Pyung-Lim Han

eLife 10: PubMed34121659

2021

Extracellular signal-regulated kinase mediates chromatin rewiring and lineage transformation in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Inoue,Ana Nikolic,Dylan Farnsworth,Rocky Shi,Fraser D Johnson,Alvin Liu,Marc Ladanyi,Romel Somwar,Marco Gallo,William W Lockwood

Molecular carcinogenesis 59:73-86 PubMed31670855

2019

ETV4 overexpression promotes progression of non-small cell lung cancer by upregulating PXN and MMP1 transcriptionally.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Xiaosong Ding,Bei Liu,Minglei Li,Ying Chang,Haitao Shen,Shelly M Xie,Lingxiao Xing,Yuehong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pea3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Pea3 antibody (AB189826)

Western blot - Anti-Pea3 antibody (AB189826)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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