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AB236503

Anti-Pea3 antibody [OTI5C11]

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(2 Publications)

Mouse Monoclonal Pea3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ETV4 aa 1-300.

View Alternative Names

E1AF, PEA3, ETV4, ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3

1 Images
Western blot - Anti-Pea3 antibody [OTI5C11] (AB236503)
  • WB

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Western blot - Anti-Pea3 antibody [OTI5C11] (AB236503)

All lanes:

Western blot - Anti-Pea3 antibody [OTI5C11] (ab236503) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, cell lysate at 5 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY Pea3, cell lysate at 5 µg

Predicted band size: 54 kDa,65 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI5C11

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ETV4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P43268

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pea3 also called PEA3 or ETV4 functions as a transcription factor from the ETS family. It has a mass of approximately 55 kDa. This protein regulates gene expression by binding to specific DNA sequences. Pea3 is mainly expressed in the brain lung and liver tissues where it influences the transcription of a range of target genes. It plays an important role in mediating cellular responses to extracellular signals.
Biological function summary

Pea3 influences key processes like cell proliferation differentiation and migration. It often operates within larger protein complexes to execute these roles. Pea3 has been shown to regulate genes involved in development and oncogenic processes by interacting with co-factors in transcriptional complexes. By activating or repressing the transcription of specific genes Pea3 contributes to the dynamic regulation of cellular environments.

Pathways

Several intracellular signaling pathways involve Pea3 in integrating cellular responses to external stimuli. Notably Pea3 plays a role in the MAPK/ERK pathway where it works alongside proteins like ERK and other transcription factors to mediate cell growth and differentiation. Pea3 also participates in the PI3K/AKT pathway influencing cell survival and metabolism. In these pathways Pea3 acts as a bridge linking extracellular signals to transcriptional outputs.

Pea3 connects to cancer progression and metastasis. Overexpression of Pea3 is associated with various cancers including breast and lung cancer where it interacts with proteins like matrix metalloproteinases (MMPs) to promote metastasis. Pea3 also links to neurological disorders by influencing genes involved in brain development and function demonstrating its widespread significance in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator (PubMed : 19307308, PubMed : 31552090). May play a role in keratinocyte differentiation (PubMed : 31552090).. (Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator; the core-binding sequence is 5'-[AC]GGA[AT]GT-3'.
See full target information ETV4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 13:3152-3174 PubMed39669999

2024

Construction and validation of a prognostic model based on oxidative stress-related genes in non-small cell lung cancer (NSCLC): predicting patient outcomes and therapy responses.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfeng Sun,Jie Lu,Wenhua Zhao,Xiaozheng Chen,Changyan Xiao,Feng Hua,Per Hydbring,Esteban C Gabazza,Alfredo Tartarone,Xiaoming Zhao,Wenfeng Yang

Disease markers 2023:8143581 PubMed36815135

2023

Transcription Activation of Rab8A by PEA3 Augments Progression of Esophagus Cancer by Activating the Wnt/-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shusheng Cai,Xiumei Liu,Weiran Gao,Lianhua Ye,Qiming Zhong,Xin Zhang
View all publications
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