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AB237686

Anti-PEAMT antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal PEAMT antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PEMT aa 1-50.

View Alternative Names

PEMPT, PNMT, PEMT, Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT2, Phospholipid methyltransferase, PLMT

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PEAMT antibody (AB237686)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PEAMT antibody (AB237686)

A549 (Human lung carcinoma cell line) cells stained for PEAMT (green) using ab237686 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

Western blot - Anti-PEAMT antibody (AB237686)
  • WB

Supplier Data

Western blot - Anti-PEAMT antibody (AB237686)

All lanes:

Western blot - Anti-PEAMT antibody (ab237686) at 1/1000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 22 kDa,58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PEMT aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UBM1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEAMT or phosphoethanolamine N-methyltransferase is an enzyme involved in phospholipid biosynthesis. This protein sometimes referred to as PEMT facilitates the conversion of phosphoethanolamine to phosphocholine donating methyl groups during the reaction. PEAMT has a molecular mass around 23 kDa. Expression of PEAMT primarily occurs in tissues with high phospholipid turnover such as the liver.
Biological function summary

Phosphoethanolamine N-methyltransferase plays a significant role in the biosynthesis of phosphatidylcholine a critical phospholipid component of cell membranes. PEAMT operates as a part of methylation processes converting phosphoethanolamine into phosphocholine through a multi-step enzymatic pathway. It does not belong to a larger protein complex but acts independently to ensure adequate phosphocholine supply which is vital for maintaining membrane integrity and function.

Pathways

PEAMT is an important participant in the CDP-choline pathway also known as the Kennedy pathway. This biological route is essential for phosphatidylcholine synthesis joining the efforts of enzymes such as choline kinase and CPT1. PEAMT's enzymatic activity ensures a steady supply of phosphocholine which ultimately integrates into phosphatidylcholine to maintain membrane structure. Its role closely connects to methylation pathways tying it with proteins involved in one-carbon metabolism.

PEAMT's activity shows a connection to liver diseases and metabolic disorders. Nonalcoholic fatty liver disease (NAFLD) often shows altered levels of phosphatidylcholine where PEAMT's altered function plays a part in its pathogenesis. Additionally metabolic syndrome shows correlation with altered phosphatidylcholine synthesis pathways where PEAMT can influence lipid profiles. Links between PEAMT and S-adenosylmethionine another key methyl donor highlight the enzyme’s impact on disorders involving methylation imbalance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PubMed : 12431977, PubMed : 15927961). Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step (PubMed : 12431977, PubMed : 15927961). Responsible for approximately 30% of hepatic PC with the CDP-choline pathway accounting for the other 70% (Probable).. Isoform 1. Catalyzes the three sequential steps of the methylation of 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine (PMME) to 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine (PDME) more efficiently than isoform 2 (PubMed : 20860552). Induces increase in PC species with longer polyunsaturated chains than isoform 2 (PubMed : 20860552).. Isoform 2. Produces a higher increase in the level of PC species containing long chains with three double bonds than isoform 1.
See full target information PEMT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:4968-4980 PubMed36056909

2022

Risk stratification and prognosis prediction based on inflammation-related gene signature in lung squamous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Zhai,Si Chen,Fangfang Duan,Junye Wang,Zerui Zhao,Yaobin Lin,Bingyu Rao,Yizhi Wang,Lie Zheng,Hao Long
View all publications
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Product promise

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