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AB235322

Anti-PECI/ECI2 antibody

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(1 Publication)

Rabbit Polyclonal PECI/ECI2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ECI2 aa 100-250.

View Alternative Names

DRS1, HCA88, PECI, ECI2, Enoyl-CoA delta isomerase 2, DRS-1, Diazepam-binding inhibitor-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Renal carcinoma antigen NY-REN-1, DBI-related protein 1, pECI

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PECI/ECI2 antibody (AB235322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PECI/ECI2 antibody (AB235322)

Paraffin-embedded human liver tissue stained for PECI/ECI2 using ab235322 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PECI/ECI2 antibody (AB235322)
  • WB

Supplier Data

Western blot - Anti-PECI/ECI2 antibody (AB235322)

All lanes:

Western blot - Anti-PECI/ECI2 antibody (ab235322) at 1/500 dilution

Lane 1:

Rat heart tissue lysate

Lane 2:

Mouse heart tissue lysate

Lane 3:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ECI2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

O75521

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/4998", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PECI also known as ECI2 or peroxisomal delta(3)delta(2)-enoyl-CoA isomerase plays an important role in the beta-oxidation of unsaturated fatty acids. This enzyme with a molecular weight of approximately 33 kDa catalyzes the conversion of cis-double bonds in unsaturated fatty acyl-CoAs turning them into trans-double bonds which are useful for subsequent beta-oxidation. PECI primarily locates to the peroxisomes and is expressed in tissues with high metabolic rates like liver and heart.
Biological function summary

PECI contributes to the metabolism of fatty acids by ensuring effective breakdown of unsaturated fatty acids in peroxisomes. The enzyme participates as a solitary player and not part of a larger protein complex. By converting cis-double bonds to the trans configuration PECI aids in stabilizing the substrate for further catabolic processing. This facilitated breakdown is critical for energy production especially during fasting when fatty acids become a significant energy source.

Pathways

PECI's mechanical function integrates into the larger pathway of fatty acid metabolism especially the peroxisomal beta-oxidation pathway. This pathway teams with enzymes such as acyl-CoA oxidase and multifunctional enzyme protein to facilitate complete fatty acid degradation. PECI's transformation of unsaturated fatty acids ensures these substrates enter the pathway's main cycle aiding in continued lipid metabolism and maintaining cellular energy balance.

Disruptions in PECI function might lead to metabolic conditions like X-linked adrenoleukodystrophy or Refsum disease which both involve impaired fatty acid oxidation. The enzyme interacts with proteins like ABCD1 a transporter associated with peroxisomal biogenesis which holds significance in adrenoleukodystrophy. Proper function and regulation of PECI remain important for managing these disorders and ensuring uninterrupted fatty acid disassembly for cellular energy requirements.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
See full target information ECI2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 21:185 PubMed33574924

2021

A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high-grade serous ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kristýna Němejcová,Michaela Bártů,Jan Hojný,Nikola Hájková,Romana Michálková,Eva Krkavcová,Ivana Stružinská,Hiep Quang Bui,Pavel Dundr,David Cibula,Kateřina Jirsová
View all publications
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Product promise

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