Rabbit Polyclonal PEDF antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINF1.
Images
Publications
Filter by
- Frontiers in bioengineering and biotechnology 10:9580722022Improvement of the bladder perfusion curative effect through tight junction protein degradation induced by magnetic temperature-sensitive hydrogels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoliang Sun,Xinhong Song,Peng Guo,Dong Zhang,Shishuai Zuo,Kang Leng,Yun Liu,Haiyang ZhangPubMed 35992356
- Oncology letters 21:2602021lncRNA GABPB1 intronic transcript 1 upregulates pigment epithelium-derived factor via miR-93 to suppress cell proliferation in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Li,Yajun Wei,Qinli Ge,Yucai Duan,Ling GuoPubMed 33664823
- International journal of molecular medicine 45:1436-14462020Pigment epithelium‑derived factor facilitates NLRP3 inflammasome activation through downregulating cytidine monophosphate kinase 2: A potential treatment strategy for missed abortion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Zhang et. al.PubMed 32323732
- Molecular medicine reports 17:6515-65252018Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis.Applications:WBReactive species:Unspecified reactive speciesWeiqing Qian,Yan Su,Yajie Zhang,Nianwei Yao,Nin Gu,Xu Zhang,Hong YinPubMed 29532868
- PloS one 8:e784412013Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing.Applications:ICC/IFReactive species:Unspecified reactive speciesClaire L Allen et. al.PubMed 24205233
- Investigative ophthalmology & visual science 54:574-842013Retinal angiogenesis in the Ins2(Akita) mouse model of diabetic retinopathy.
- Microvascular research 84:278-852012Age-related decrease in cerebrovascular-derived neuroprotective proteins: effect of acetaminophen.
- Neuroscience research 72:1-82011Pigment epithelium-derived factor (PEDF) protects cortical neurons in vitro from oxidant injury by activation of extracellular signal-regulated kinase (ERK) 1/2 and induction of Bcl-2.Applications:ELISAReactive species:Unspecified reactive speciesA Sanchez,D Tripathy,X Yin,J Luo,J Martinez,P GrammasPubMed 21946416
- Arteriosclerosis, thrombosis, and vascular biology 30:1325-322010Comparative proteomics profiling reveals role of smooth muscle progenitors in extracellular matrix production.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.184% Tris glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SERPINF1. Database link P36955
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Alternative names
PEDF, PIG35, SERPINF1, Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1
Recommended products
Rabbit Polyclonal PEDF antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINF1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.184% Tris glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SERPINF1. Database link P36955
- Purification technique
- Affinity purification Protein A
- Specificity
Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.
- Biological function summary
PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.
- Pathways
PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.
- Associated diseases and disorders
PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Kindly submitted by Dr John Wardale.Western blot - Anti-PEDF antibody (ab14993)
Lanes 1-4: Cartilage lysates from 4 osteoarthritic knee replacement donors (100 mg (wet weight) powdered cartilage/ 1 ml RIPA buffer, extracted for 24 hrs at 4°C)Lane C: Positive control (overexpressing cell line)
Anticipated band at approx 50kDa detected. Specificity of antibody allowed relatively long exposure and quite high concentrations of primary and secondary antibodies to be used with negligible background. Good results were also obtained with unreduced samples.
The additional approx 250kDa band is likely to be due to PEDF crosslinked or bound to high molecular weight matrix proteins (collagen, aggrecan) which are present in cartilage lysates. Reduction using beta mercaptoethanol reduced some, but not all high MW staining and increased the intensity of the 50kDa band.All lanes: Western blot - Anti-PEDF antibody (ab14993)
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 30s
Western blot - Anti-PEDF antibody (ab14993)
Chemiluminescence detection system.
All lanes: Western blot - Anti-PEDF antibody (ab14993) at 1 µg/mL
All lanes: Y79 (Human retinoblastoma cell line) cell lysate in RIPA buffer at 40 µg
SecondaryAll lanes: IgG (HRP) goat anti-rabbit
Predicted band size: 46 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com