JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227295

Anti-PEDF antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal PEDF antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINF1.

View Alternative Names

PEDF, PIG35, SERPINF1, Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SERPINF1. The exact immunogen used to generate this antibody is proprietary information.

P36955

style
left-column

Alternative Products

Primary Antibodies

AB203033

Anti-PEDF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEDF antibody (AB203033)

  • 0
  • 0
Primary Antibodies

AB157207

Anti-PEDF antibody [EPR9826(B)]

RabMAb|Recombinant

Western blot - Anti-PEDF antibody [EPR9826(B)] (AB157207)

  • 0
  • 0
Primary Antibodies

AB14993

Anti-PEDF antibody

Western blot - Anti-PEDF antibody (AB14993)

  • 4
  • 1

Complementary Products

Assay Kits

AB246535

Human PEDF ELISA Kit

Recombinant|SimpleStep

ELISA - Human PEDF ELISA Kit (AB246535)

  • 0
  • 0
Primary Antibodies

AB32570

Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (AB32570)

  • 4
  • 31
Primary Antibodies

AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

  • 0
  • 0
Primary Antibodies

AB283346

Anti-Iba1 antibody [EPR16589] - Microglia marker - Rat IgG2a (Chimeric)

BOND RX™ Validated|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] - Microglia marker - Rat IgG2a (Chimeric) (AB283346)

  • 5
  • 3
Primary Antibodies

AB52917

Anti-VEGFA antibody [EP1176Y] - C-terminal

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] - C-terminal (AB52917)

  • 5
  • 9
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.
Biological function summary

PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.

Pathways

PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.

PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
See full target information SERPINF1
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, metabolic syndrome and obesity : targets and therapy 18:217-231 PubMed39896707

2025

PEDF Overexpression Ameliorates Cardiac Lipotoxicity in Diabetic Cardiomyopathy via Regulation of Energy Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tuohua Mao,Ye Wang

Cell 187:914-930.e20 PubMed38280375

2024

Gut-liver axis calibrates intestinal stem cell fitness.

Applications

Unspecified application

Species

Unspecified reactive species

Girak Kim,Zuojia Chen,Jian Li,Jialie Luo,Felipe Castro-Martinez,Jan Wisniewski,Kairong Cui,Yan Wang,Jialei Sun,Xiaobai Ren,Susan E Crawford,S Patricia Becerra,Jimin Zhu,Taotao Liu,Sui Wang,Keji Zhao,Chuan Wu

Frontiers in oncology 11:784777 PubMed34988020

2021

Hypoxia-Induced Intracellular and Extracellular Heat Shock Protein gp96 Increases Paclitaxel-Resistance and Facilitates Immune Evasion in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Jiguang Han,Jian Huang,Shangziyan Li,Hui Pang

Human cell 34:1093-1102 PubMed33768511

2021

Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes and PEDF secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhui Zhang,Wenya Fan,Lingling Neng,Bei Chen,Bin Zuo,Wei Lu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com