AB307083

Anti-PEDF antibody [EPR25141-150]

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal PEDF antibody. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PEDF, PIG35, SERPINF1, Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PEDF antibody [EPR25141-150] (AB307083)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PEDF antibody [EPR25141-150] (AB307083)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling PEDF with ab307083 at 1/100 dilution (5.76 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Negative control : MCF7 (PMID : 16740777). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PEDF antibody [EPR25141-150] (AB307083)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling PEDF with ab307083 at 1/100 dilution (5.76 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Low expression control : PC-3 (PMID : 16740777). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

Flow Cytometry (Intracellular) - Anti-PEDF antibody [EPR25141-150] (AB307083)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PEDF antibody [EPR25141-150] (AB307083)

Flow cytometric analysis of 80% methanol fixed 0.1% Tween-20 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labeling PEDF with ab307083 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression control : PC-3 (PMID : 16740777).

Carrier free

Anti-PEDF antibody [EPR25141-150] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25141-150

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.

Biological function summary

PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.

Pathways

PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.

PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

See full target information SERPINF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:178 PubMed39034417

2024

PEDF-34 attenuates neurological deficit and suppresses astrocyte-dependent neuroinflammation by modulating astrocyte polarization via 67LR/JNK/STAT1 signaling pathway after subarachnoid hemorrhage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wu,Yanchao Liu,Qiuguang He,Guangnan Ao,Ningbo Xu,Wangqing He,Xiao Liu,Lei Huang,Qian Yu,Hideki Kanamaru,Siyuan Dong,Shiyi Zhu,Ye Yuan,Mingyang Han,Yeping Ling,Lu Liu,Chenyu Wu,You Zhou,Prativa Sherchan,Jerry J Flores,Jiping Tang,Xionghui Chen,Xuying He,John H Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PEDF antibody [EPR25141-150]

Immunocytochemistry/ Immunofluorescence - Anti-PEDF antibody [EPR25141-150] (AB307083)

Immunocytochemistry/ Immunofluorescence - Anti-PEDF antibody [EPR25141-150] (AB307083)

Flow Cytometry (Intracellular) - Anti-PEDF antibody [EPR25141-150] (AB307083)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise