AB157207

Anti-PEDF antibody [EPR9826(B)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal PEDF antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PEDF, PIG35, SERPINF1, Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1

1 Images

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Western blot - Anti-PEDF antibody [EPR9826(B)] (AB157207)

All lanes:

Western blot - Anti-PEDF antibody [EPR9826(B)] (ab157207) at 1/1000 dilution

Lane 1:

Human plasma at 10 µg

Lane 2:

Human serum at 10 µg

Lane 3:

Y79 (Human retinoblastoma cell line) lysate at 10 µg

Lane 4:

A549 (Human lung carcinoma cell line) lysate at 10 µg

Lane 5:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate at 10 µg

Lane 6:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) lysate at 10 µg

Predicted band size: 46 kDa

false

Carrier free

Anti-PEDF antibody [EPR9826(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9826(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100 - 1/500", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.

Biological function summary

PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.

Pathways

PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.

PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

See full target information SERPINF1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0279191 PubMed36574366

2022

Sonodynamic therapy suppresses matrix collagen degradation in vulnerable atherosclerotic plaque by modulating caspase 3 - PEDF/HIF-1α - MMP-2/MMP-9 signaling in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfeng Tian,Siqi Sheng,Weiwei Gao,Jianting Yao,Ye Tian

International journal of molecular sciences 22: PubMed33810153

2021

A ROCK Inhibitor Promotes Graft Survival during Transplantation of iPS-Cell-Derived Retinal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masaaki Ishida,Sunao Sugita,Kenichi Makabe,Shota Fujii,Yoko Futatsugi,Hiroyuki Kamao,Suguru Yamasaki,Noriko Sakai,Akiko Maeda,Michiko Mandai,Masayo Takahashi

Methods in molecular biology (Clifton, N.J.) 1994:31-39 PubMed31124102

2019

Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Janssen,Lamiaa Bahnassawy,Claudia Kiefer,Jürgen Korffmann,Georg C Terstappen,Viktor Lakics,Miroslav Cik,Peter Reinhardt

Nature 535:164-8 PubMed27383988

2016

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zhang,Jonathan J Miner,Matthew J Gorman,Keiko Rausch,Holly Ramage,James P White,Adam Zuiani,Ping Zhang,Estefania Fernandez,Qiang Zhang,Kimberly A Dowd,Theodore C Pierson,Sara Cherry,Michael S Diamond

Cancer biology & therapy 17:507-14 PubMed26987032

2016

Metformin inhibits prostate cancer cell proliferation, migration, and tumor growth through upregulation of PEDF expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowan Chen,Chenli Li,Tiantian He,Jiating Mao,Chunmei Li,Jianxin Lyu,Qing H Meng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PEDF antibody [EPR9826(B)]

Western blot - Anti-PEDF antibody [EPR9826(B)] (AB157207)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise