Rabbit Polyclonal PEF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PEF1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PEF1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBV8
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).
Alternative names
ABP32, UNQ1845/PRO3573, PEF1, Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1
Recommended products
Rabbit Polyclonal PEF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PEF1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PEF1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBV8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEF1 also known as Peflin is a calcium-binding protein with a molecular weight of approximately 29 kDa. This protein is situated in the cytoplasm and localizes predominantly in the brain and heart tissues. PEF1 is part of the penta-EF-hand protein family characterized by its EF-hand calcium-binding motifs which allow it to sense calcium ion concentrations and mediate cellular processes.
- Biological function summary
PEF1 plays a significant role in calcium-dependent signaling pathways. It frequently partners with sorcin to form a heterodimeric complex that regulates intracellular calcium levels critical for cellular homeostasis. By binding calcium ions PEF1 modulates the function of various calcium-dependent proteins facilitating communication between cellular signaling mechanisms.
- Pathways
PEF1 integrates into cellular signaling mechanisms by modulating the availability of free calcium ions. It is specifically active in the calcineurin/NFAT pathway important for T-cell activation and myocardial function. PEF1's interaction with sorcin allows it to modulate the activity of other proteins like ryanodine receptors which are important for calcium release from the sarcoplasmic reticulum.
- Associated diseases and disorders
PEF1 has been linked to cardiac hypertrophy and neurodegenerative conditions like Alzheimer's disease. PEF1's dysregulation can disrupt calcium homeostasis contributing to these conditions. In cardiac hypertrophy for instance abnormal PEF1 levels affect calcineurin/NFAT signaling leading to pathological heart growth. In Alzheimer's disease altered PEF1 function may be associated with the perturbation of calcium-regulated processes critical for neuronal survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PEF1 antibody (ab96132)
All lanes: Western blot - Anti-PEF1 antibody (ab96132) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PEF1 antibody (ab96132)
ab96132, at 1/200 staining HeLa cells by Immunofluorescence. Lower image merged with DNA probe.
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