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AB96132

Anti-PEF1 antibody

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(1 Publication)

Rabbit Polyclonal PEF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PEF1.

View Alternative Names

ABP32, UNQ1845/PRO3573, PEF1, Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PEF1 antibody (AB96132)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PEF1 antibody (AB96132)

ab96132, at 1/200 staining HeLa cells by Immunofluorescence. Lower image merged with DNA probe.

Western blot - Anti-PEF1 antibody (AB96132)
  • WB

Unknown

Western blot - Anti-PEF1 antibody (AB96132)

All lanes:

Western blot - Anti-PEF1 antibody (ab96132) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PEF1. The exact immunogen used to generate this antibody is proprietary information.

Q9UBV8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEF1 also known as Peflin is a calcium-binding protein with a molecular weight of approximately 29 kDa. This protein is situated in the cytoplasm and localizes predominantly in the brain and heart tissues. PEF1 is part of the penta-EF-hand protein family characterized by its EF-hand calcium-binding motifs which allow it to sense calcium ion concentrations and mediate cellular processes.
Biological function summary

PEF1 plays a significant role in calcium-dependent signaling pathways. It frequently partners with sorcin to form a heterodimeric complex that regulates intracellular calcium levels critical for cellular homeostasis. By binding calcium ions PEF1 modulates the function of various calcium-dependent proteins facilitating communication between cellular signaling mechanisms.

Pathways

PEF1 integrates into cellular signaling mechanisms by modulating the availability of free calcium ions. It is specifically active in the calcineurin/NFAT pathway important for T-cell activation and myocardial function. PEF1's interaction with sorcin allows it to modulate the activity of other proteins like ryanodine receptors which are important for calcium release from the sarcoplasmic reticulum.

PEF1 has been linked to cardiac hypertrophy and neurodegenerative conditions like Alzheimer's disease. PEF1's dysregulation can disrupt calcium homeostasis contributing to these conditions. In cardiac hypertrophy for instance abnormal PEF1 levels affect calcineurin/NFAT signaling leading to pathological heart growth. In Alzheimer's disease altered PEF1 function may be associated with the perturbation of calcium-regulated processes critical for neuronal survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed : 27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed : 27716508). Its role in the heterodimer formed with PDCD6 is however unclear : some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed : 27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed : 11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).
See full target information PEF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 37: PubMed29661885

2018

The long non-coding RNA promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ioanna Pavlaki,Farah Alammari,Bin Sun,Neil Clark,Tamara Sirey,Sheena Lee,Dan J Woodcock,Chris P Ponting,Francis G Szele,Keith W Vance
View all publications
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Product promise

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