AB137127

Anti-PEF1 antibody [EPR9310]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal PEF1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

ABP32, UNQ1845/PRO3573, PEF1, Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1

2 Images

Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] (AB137127)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] (AB137127)

Intracellular flow cytometric analysis of permeabilized 293T cells labelling PEF1 with ab137127 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-PEF1 antibody [EPR9310] (AB137127)

All lanes:

Western blot - Anti-PEF1 antibody [EPR9310] (ab137127) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HL60 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 30 kDa

false

Carrier free

Anti-PEF1 antibody [EPR9310] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9310

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEF1 also known as Peflin is a calcium-binding protein with a molecular weight of approximately 29 kDa. This protein is situated in the cytoplasm and localizes predominantly in the brain and heart tissues. PEF1 is part of the penta-EF-hand protein family characterized by its EF-hand calcium-binding motifs which allow it to sense calcium ion concentrations and mediate cellular processes.

Biological function summary

PEF1 plays a significant role in calcium-dependent signaling pathways. It frequently partners with sorcin to form a heterodimeric complex that regulates intracellular calcium levels critical for cellular homeostasis. By binding calcium ions PEF1 modulates the function of various calcium-dependent proteins facilitating communication between cellular signaling mechanisms.

Pathways

PEF1 integrates into cellular signaling mechanisms by modulating the availability of free calcium ions. It is specifically active in the calcineurin/NFAT pathway important for T-cell activation and myocardial function. PEF1's interaction with sorcin allows it to modulate the activity of other proteins like ryanodine receptors which are important for calcium release from the sarcoplasmic reticulum.

PEF1 has been linked to cardiac hypertrophy and neurodegenerative conditions like Alzheimer's disease. PEF1's dysregulation can disrupt calcium homeostasis contributing to these conditions. In cardiac hypertrophy for instance abnormal PEF1 levels affect calcineurin/NFAT signaling leading to pathological heart growth. In Alzheimer's disease altered PEF1 function may be associated with the perturbation of calcium-regulated processes critical for neuronal survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed : 27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed : 27716508). Its role in the heterodimer formed with PDCD6 is however unclear : some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed : 27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed : 11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).

See full target information PEF1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:585-597.e11 PubMed35120648

2022

Co-adaptor driven assembly of a CUL3 E3 ligase complex.

Applications

Unspecified application

Species

Unspecified reactive species

David Akopian,Colleen A McGourty,Michael Rapé

FASEB bioAdvances 4:43-59 PubMed35024572

2022

Interplay in neural functions of cell adhesion molecule close homolog of L1 (CHL1) and Programmed Cell Death 6 (PDCD6).

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Loers,Thomas Theis,Helen Baixia Hao,Ralf Kleene,Sanjana Arsha,Nina Samuel,Neha Arsha,Wise Young,Melitta Schachner

Nature 586:452-456 PubMed32814905

2020

Structural basis for dimerization quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Elijah L Mena,Predrag Jevtić,Basil J Greber,Christine L Gee,Brandon G Lew,David Akopian,Eva Nogales,John Kuriyan,Michael Rape

Cell 167:525-538.e14 PubMed27716508

2016

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Applications

Unspecified application

Species

Unspecified reactive species

Colleen A McGourty,David Akopian,Carolyn Walsh,Amita Gorur,Achim Werner,Randy Schekman,Diana Bautista,Michael Rape

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PEF1 antibody [EPR9310]

Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] (AB137127)

Western blot - Anti-PEF1 antibody [EPR9310] (AB137127)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise