Rabbit Recombinant Monoclonal PEF1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (AB248836), expandable thumbnail](https://content.abcam.com/products/images/pef1-antibody-epr9310-bsa-and-azide-free-ab248836--western-blot-img105127.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (AB248836)")
![Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (AB248836), expandable thumbnail](https://content.abcam.com/products/images/pef1-antibody-epr9310-bsa-and-azide-free-ab248836--flow-cytometry-intracellular-img29768.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (AB248836)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).
Alternative names
ABP32, UNQ1845/PRO3573, PEF1, Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1
Recommended products
Rabbit Recombinant Monoclonal PEF1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9310
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248836 is the carrier-free version of Anti-PEF1 antibody [EPR9310] ab137127.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEF1 also known as Peflin is a calcium-binding protein with a molecular weight of approximately 29 kDa. This protein is situated in the cytoplasm and localizes predominantly in the brain and heart tissues. PEF1 is part of the penta-EF-hand protein family characterized by its EF-hand calcium-binding motifs which allow it to sense calcium ion concentrations and mediate cellular processes.
- Biological function summary
PEF1 plays a significant role in calcium-dependent signaling pathways. It frequently partners with sorcin to form a heterodimeric complex that regulates intracellular calcium levels critical for cellular homeostasis. By binding calcium ions PEF1 modulates the function of various calcium-dependent proteins facilitating communication between cellular signaling mechanisms.
- Pathways
PEF1 integrates into cellular signaling mechanisms by modulating the availability of free calcium ions. It is specifically active in the calcineurin/NFAT pathway important for T-cell activation and myocardial function. PEF1's interaction with sorcin allows it to modulate the activity of other proteins like ryanodine receptors which are important for calcium release from the sarcoplasmic reticulum.
- Associated diseases and disorders
PEF1 has been linked to cardiac hypertrophy and neurodegenerative conditions like Alzheimer's disease. PEF1's dysregulation can disrupt calcium homeostasis contributing to these conditions. In cardiac hypertrophy for instance abnormal PEF1 levels affect calcineurin/NFAT signaling leading to pathological heart growth. In Alzheimer's disease altered PEF1 function may be associated with the perturbation of calcium-regulated processes critical for neuronal survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (ab248836), expandable thumbnail](https://content.abcam.com/products/images/pef1-antibody-epr9310-bsa-and-azide-free-ab248836--western-blot-img105127.jpg "Western blot - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (ab248836)")
Western blot - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (ab248836)
This data was developed using Anti-PEF1 antibody [EPR9310] ab137127, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PEF1 antibody [EPR9310] (Anti-PEF1 antibody [EPR9310] ab137127) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: HL60 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
![Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (ab248836), expandable thumbnail](https://content.abcam.com/products/images/pef1-antibody-epr9310-bsa-and-azide-free-ab248836--flow-cytometry-intracellular-img29768.jpg "Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (ab248836)")
Flow Cytometry (Intracellular) - Anti-PEF1 antibody [EPR9310] - BSA and Azide free (ab248836)
This data was developed using Anti-PEF1 antibody [EPR9310] ab137127, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized 293T cells labelling PEF1 with Anti-PEF1 antibody [EPR9310] ab137127 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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