Skip to main content

Rabbit Polyclonal PEG10/EDR antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PEG10 aa 300-400.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-PEG10/EDR antibody (AB226370), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human PEG10 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q86TG7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IP
Human
Tested
Chimpanzee
Predicted
Dog
Predicted
Giant Panda
Predicted
Rhesus monkey
Predicted

Tested
Tested

Species

Human

Dilution info

2-10 µg

Notes

-

Predicted
Predicted

Species

Dog, Chimpanzee, Rhesus monkey, Giant Panda

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Retrotransposon-derived protein that binds its own mRNA and self-assembles into virion-like capsids (PubMed:34413232). Forms virion-like extracellular vesicles that encapsulate their own mRNA and are released from cells, enabling intercellular transfer of PEG10 mRNA (PubMed:34413232). Binds its own mRNA in the 5'-UTR region, in the region near the boundary between the nucleocapsid (NC) and protease (PRO) coding sequences and in the beginning of the 3'-UTR region (PubMed:34413232). Involved in placenta formation: required for trophoblast stem cells differentiation (By similarity). Involved at the immediate early stage of adipocyte differentiation (By similarity). Overexpressed in many cancers and enhances tumor progression: promotes cell proliferation by driving cell cycle progression from G0/G1 (PubMed:12810624, PubMed:16423995, PubMed:26235627, PubMed:28193232). Enhances cancer progression by inhibiting the TGF-beta signaling, possibly via interaction with the TGF-beta receptor ACVRL1 (PubMed:15611116, PubMed:26235627, PubMed:30094509). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter; additional evidences are however required to confirm this result (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal PEG10/EDR antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PEG10 aa 300-400.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human PEG10 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q86TG7
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab226370 was affinity purified using an epitope specific to PEG10/EDR immobilized on solid support.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab226370 has not performed satisfactorily when used for WB of PEG10/EDR in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PEG10/EDR.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PEG10 also known as EDR2 is a paternally expressed imprinted gene that encodes the PEG10 protein. The PEG10 protein has a molecular mass of approximately 150 kDa. It shows expression most notably in the placenta during development but also in other tissues like the liver and brain. The protein contains two open reading frames that produce different products via a frameshift mechanism similar to retroviral proteins.

Biological function summary

The PEG10 protein plays multiple roles especially in cell proliferation apoptosis inhibition and promoting cell survival. It can be part of larger protein complexes that modulate cellular responses to stress and developmental cues. The protein's multifaceted activities are important during embryogenesis where it supports trophoblast development and placental formation.

Pathways

PEG10 interacts with signaling cascades such as the TGF-beta signaling pathway and is involved in cell cycle regulation. PEG10 works alongside proteins like paternally expressed genes that connect with TGF-beta pathways influencing cellular differentiation and growth. Its integration in these pathways highlights its role in managing cellular environments and responses to cellular stress signals.

Associated diseases and disorders

PEG10 expression over-activation has associations with cancer especially hepatocellular carcinoma and pancreatic cancer. In these contexts PEG10 interacts with proteins like H3L3 contributing to oncogenic pathways that drive tumor cell proliferation and survival. Elevated levels of PEG10 can serve as potential biomarkers for certain cancers suggesting their role in disease progression and possible therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunoprecipitation - Anti-PEG10/EDR antibody (ab226370), expandable thumbnail

    Immunoprecipitation - Anti-PEG10/EDR antibody (ab226370)

    PEG10/EDR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226370 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226370 at 0.4 μg/ml.

    Lane 1: ab226370 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

    All lanes: Immunoprecipitation - Anti-PEG10/EDR antibody (ab226370)

    Predicted band size: 80 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com