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AB13812

Anti-Pellino 1 antibody

4

(5 Reviews)

|

(5 Publications)

Rabbit Polyclonal Pellino 1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PELI1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PRISM, PELI1, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-1, Pellino-related intracellular-signaling molecule, RING-type E3 ubiquitin transferase pellino homolog 1

1 Images
Western blot - Anti-Pellino 1 antibody (AB13812)
  • WB

Unknown

Western blot - Anti-Pellino 1 antibody (AB13812)

Western blot analysis of Pellino1 in human Ramos cell lysate (A) and mouse Raw cell lysate (B) using ab13812 at 1/1000

All lanes:

Western blot - Anti-Pellino 1 antibody (ab13812) at 1/1000 dilution

Lane 1:

Lysates prepared from human Ramos cell line

Lane 2:

Lysates prepared from mouse Raw cell line

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PELI1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96FA3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
This antibody is affinity purified.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pellino 1 is an E3 ubiquitin ligase important in signaling pathways linked to immune response and inflammation. It is also known as RNF232 and has a molecular weight of approximately 41 kDa. You can find expression of Pellino 1 in various tissues including immune cells like macrophages and dendritic cells. Its activity relies on its ability to add ubiquitin moieties to target proteins a process essential for tagging these proteins for cellular processes like degradation or trafficking.
Biological function summary

Pellino 1 plays a major role in immune signaling networks. It acts as an adaptor and signal transducer which facilitates the recruitment and regulation of other signaling molecules. It often forms part of a signaling complex within the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) pathways. The complex assembly enables Pellino 1 to modulate innate immune responses by influencing downstream signaling events that elevate inflammatory cytokine production and immune cell activation.

Pathways

Pellino 1 participates significantly in the TLR and IL-1R signaling pathways. These pathways are critical for activating nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways enabling transcription of immune-related genes. In the TLR pathway Pellino 1 functions alongside proteins such as IRAK1 modulating pathway outcomes by regulating the ubiquitination of downstream signaling components.

Pellino 1 frequently associates with autoimmune diseases and chronic inflammatory conditions. Its role in modulating immune responses links it to conditions such as rheumatoid arthritis where it influences the inflammatory response through interactions with key proteins like TRAF6. Aberrations in Pellino 1 function or expression have also connections to systemic lupus erythematosus as its regulation of NF-κB signaling affects autoantibody production and inflammatory cell survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed : 12496252, PubMed : 17675297, PubMed : 29883609, PubMed : 30952868). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 (PubMed : 12496252, PubMed : 17675297). Acts as a positive regulator of inflammatory response in microglia through activation of NF-kappa-B and MAP kinase (By similarity). Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed : 12496252, PubMed : 17675297). Conjugates 'Lys-63'-linked ubiquitin chains to the adapter protein ASC/PYCARD, which in turn is crucial for NLRP3 inflammasome activation (PubMed : 34706239). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed : 29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed : 29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed : 29883609). Following phosphorylation by ATM, catalyzes 'Lys-63'-linked ubiquitination of NBN, promoting DNA repair via homologous recombination (PubMed : 30952868). Negatively regulates activation of the metabolic mTORC1 signaling pathway by mediating 'Lys-63'-linked ubiquitination of mTORC1-inhibitory protein TSC1 and thereby promoting TSC1/TSC2 complex stability (PubMed : 33215753).
See full target information PELI1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40049731

2025

MDC1 mediates Pellino recruitment to sites of DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Mònica Torres Esteban,Matthew J Stewart,Eilis Bragginton,Alice Meroni,Annica Pellizzari,Alain Jeanrenaud,Stephen J Smerdon,Manuel Stucki

Journal of the American Heart Association 7:e007601 PubMed30371196

2018

Disruption of VEGF Mediated Flk-1 Signaling Leads to a Gradual Loss of Vessel Health and Cardiac Function During Myocardial Infarction: Potential Therapy With Pellino-1.

Applications

Unspecified application

Species

Unspecified reactive species

Mahesh Thirunavukkarasu,Vaithinathan Selvaraju,Mandip Joshi,Vladimir Coca-Soliz,Leonidas Tapias,IbnalWalid Saad,Craig Fournier,Aaftab Husain,Jacob Campbell,Siu-Pok Yee,Juan A Sanchez,J Alexander Palesty,David W McFadden,Nilanjana Maulik

Microcirculation (New York, N.Y. : 1994) 24: PubMed28177171

2017

Increased survivability of ischemic skin flap tissue in Flk-1 mice by Pellino-1 intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Chandra K Rednam,Rickesha L Wilson,Vaithinathan Selvaraju,Muhammad T Rishi,Mahesh Thirunavukkarasu,Vladimir Coca-Soliz,Rajesh Lakshmanan,John A Palesty,David W McFadden,Nilanjana Maulik

eNeuro 3: PubMed27257617

2016

Bid Promotes K63-Linked Polyubiquitination of Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF6) and Sensitizes to Mutant SOD1-Induced Proinflammatory Signaling in Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Sinéad Kinsella,Hans-Georg König,Jochen H M Prehn

Carcinogenesis 34:1216-23 PubMed23385064

2013

Interaction of the oncogenic miR-21 microRNA and the p53 tumor suppressor pathway.

Applications

Unspecified application

Species

Mouse

Xiaodong Ma,Saibyasachi N Choudhury,Xiang Hua,Zhongping Dai,Yong Li
View all publications
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