Rabbit Polyclonal Pellino 1 antibody. C-terminal. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PELI1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PELI1. The exact immunogen used to generate this antibody is proprietary information. Database link Q96FA3
Reactivity data
Select an application| ICC | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:12496252, PubMed:17675297, PubMed:29883609, PubMed:30952868). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 (PubMed:12496252, PubMed:17675297). Acts as a positive regulator of inflammatory response in microglia through activation of NF-kappa-B and MAP kinase (By similarity). Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:12496252, PubMed:17675297). Conjugates 'Lys-63'-linked ubiquitin chains to the adapter protein ASC/PYCARD, which in turn is crucial for NLRP3 inflammasome activation (PubMed:34706239). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed:29883609). Following phosphorylation by ATM, catalyzes 'Lys-63'-linked ubiquitination of NBN, promoting DNA repair via homologous recombination (PubMed:30952868). Negatively regulates activation of the metabolic mTORC1 signaling pathway by mediating 'Lys-63'-linked ubiquitination of mTORC1-inhibitory protein TSC1 and thereby promoting TSC1/TSC2 complex stability (PubMed:33215753).
Alternative names
PRISM, PELI1, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-1, Pellino-related intracellular-signaling molecule, RING-type E3 ubiquitin transferase pellino homolog 1
Recommended products
Rabbit Polyclonal Pellino 1 antibody. C-terminal. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PELI1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PELI1. The exact immunogen used to generate this antibody is proprietary information. Database link Q96FA3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pellino 1 is an E3 ubiquitin ligase important in signaling pathways linked to immune response and inflammation. It is also known as RNF232 and has a molecular weight of approximately 41 kDa. You can find expression of Pellino 1 in various tissues including immune cells like macrophages and dendritic cells. Its activity relies on its ability to add ubiquitin moieties to target proteins a process essential for tagging these proteins for cellular processes like degradation or trafficking.
- Biological function summary
Pellino 1 plays a major role in immune signaling networks. It acts as an adaptor and signal transducer which facilitates the recruitment and regulation of other signaling molecules. It often forms part of a signaling complex within the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) pathways. The complex assembly enables Pellino 1 to modulate innate immune responses by influencing downstream signaling events that elevate inflammatory cytokine production and immune cell activation.
- Pathways
Pellino 1 participates significantly in the TLR and IL-1R signaling pathways. These pathways are critical for activating nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways enabling transcription of immune-related genes. In the TLR pathway Pellino 1 functions alongside proteins such as IRAK1 modulating pathway outcomes by regulating the ubiquitination of downstream signaling components.
- Associated diseases and disorders
Pellino 1 frequently associates with autoimmune diseases and chronic inflammatory conditions. Its role in modulating immune responses links it to conditions such as rheumatoid arthritis where it influences the inflammatory response through interactions with key proteins like TRAF6. Aberrations in Pellino 1 function or expression have also connections to systemic lupus erythematosus as its regulation of NF-κB signaling affects autoantibody production and inflammatory cell survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Pellino 1 antibody - C-terminal (ab189953)
Lane 1: Western blot - Anti-Pellino 1 antibody - C-terminal (ab189953) at 1 µg/mL
Lane 2: Western blot - Anti-Pellino 1 antibody - C-terminal (ab189953) at 2 µg/mL
All lanes: Human liver tissue lysate
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pellino 1 antibody - C-terminal (ab189953)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling Pellino 1 with ab189953 at 5 μg/ml.
Immunocytochemistry - Anti-Pellino 1 antibody - C-terminal (ab189953)
Immunocytochemical analysis of HepG2 cells labeling Pellino 1 with ab189953 at 2.5 μg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-Pellino 1 antibody - C-terminal (ab189953)
Immunofluorescent analysis of HepG2 cells labeling Pellino 1 with ab189953 at 20 μg/ml.
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