AB251152

Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Pellino 1 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

PRISM, PELI1, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-1, Pellino-related intracellular-signaling molecule, RING-type E3 ubiquitin transferase pellino homolog 1

4 Images

Supplier Data

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

This data was developed using ab192833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression of Pellino 1 can be induced by LPS (PMID : 21204785).

All lanes:

Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (<a href='/en-us/products/primary-antibodies/pellino-1-pellino-2-antibody-epr19282-ab192833'>ab192833</a>) at 1/1000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 3:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 1 μg/ml LPS for 6 hours, whole cell lysate at 20 µg

Lane 4:

Untreated PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma cell line) treated with 1 μg/ml LPS for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

This data was developed using ab192833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (<a href='/en-us/products/primary-antibodies/pellino-1-pellino-2-antibody-epr19282-ab192833'>ab192833</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

This data was developed using ab192833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 1 second; Lanes 2-3 : 1 minute.

Human Pellino 1 full length recombinant protein contains aa1-418 with a His-Tag^®. Human Pellino 2 full length recombinant protein contains aa1-420 with a His-Tag^®. Human Pellino 3 full length recombinant protein contains aa1-469 with a His-Tag^®. All three recombinant proteins were made in-house.

All lanes:

Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (<a href='/en-us/products/primary-antibodies/pellino-1-pellino-2-antibody-epr19282-ab192833'>ab192833</a>) at 1/5000 dilution

Lane 1:

Human Pellino 1 full length recombinant protein at 0.01 µg

Lane 2:

Human Pellino 2 full length recombinant protein at 0.01 µg

Lane 3:

Human Pellino 3 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Unknown

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

This data was developed using ab192833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (<a href='/en-us/products/primary-antibodies/pellino-1-pellino-2-antibody-epr19282-ab192833'>ab192833</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 2:

Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19282

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251152 is the carrier-free version of ab192833.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pellino 1 and Pellino 2 are E3 ubiquitin ligases and known as PELI1 and PELI2 respectively. PELI1 has a mass of approximately 42 kDa while PELI2 has a similar size around 40 kDa. These proteins are expressed in various tissues including immune cells where they play important roles in cellular signaling processes. Both Pellino proteins contain RING-like domains which facilitate their role in protein ubiquitination a process tagging proteins for degradation or modulating their function.

Biological function summary

Pellino proteins are involved in immune response modulation. They influence signaling pathways such as Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) pathways. Pellino proteins participate in forming complexes with other signaling proteins assisting in the propagation of inflammatory responses. Their ability to link with components like IRAK1 and TRAF6 helps regulate these immune signaling cascades critical for innate immunity.

Pathways

Pellino proteins are involved in the TLR and IL-1R pathways which are key to innate immune responses. These pathways often include the activation of NF-kB a major transcription factor that regulates immune and inflammatory responses. Pellino proteins interact with signaling proteins like IRAK1 leading to the further propagation of signals that culminate in the activation of NF-kB and the production of pro-inflammatory cytokines.

Pellino proteins are connected to conditions like rheumatoid arthritis and systemic lupus erythematosus. In rheumatoid arthritis aberrant signaling pathways involving Pellino proteins contribute to chronic inflammation and tissue damage. Through their interaction with IRAK1 Pellino proteins can affect downstream signaling that exacerbates auto-inflammatory responses. Their dysregulation can therefore link directly to mechanisms underpinning these autoimmune diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed : 12496252, PubMed : 17675297, PubMed : 29883609, PubMed : 30952868). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 (PubMed : 12496252, PubMed : 17675297). Acts as a positive regulator of inflammatory response in microglia through activation of NF-kappa-B and MAP kinase (By similarity). Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed : 12496252, PubMed : 17675297). Conjugates 'Lys-63'-linked ubiquitin chains to the adapter protein ASC/PYCARD, which in turn is crucial for NLRP3 inflammasome activation (PubMed : 34706239). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed : 29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed : 29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed : 29883609). Following phosphorylation by ATM, catalyzes 'Lys-63'-linked ubiquitination of NBN, promoting DNA repair via homologous recombination (PubMed : 30952868). Negatively regulates activation of the metabolic mTORC1 signaling pathway by mediating 'Lys-63'-linked ubiquitination of mTORC1-inhibitory protein TSC1 and thereby promoting TSC1/TSC2 complex stability (PubMed : 33215753).

See full target information PELI1

Additional targets

PELI2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (AB251152)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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