AB154335

Anti-PELO antibody

Name: Anti-PELO antibody (ab154335) | Abcam
Brand: Abcam Limited
SKU: AB154335

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(2 Publications)

Rabbit Polyclonal PELO antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PELO aa 1-300.

View Alternative Names

CGI-17, PELO, Protein pelota homolog, hPelota, Protein Dom34 homolog

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody (AB154335)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody (AB154335)

Immunohistochemical analysis of paraffin-embedded Human HBL435 xenograft tissue, labeling PELO with ab154335 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PELO antibody (AB154335)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PELO antibody (AB154335)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells (4% at RT; 15mins), labeling PELO with ab154335 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.

Supplier Data

Western blot - Anti-PELO antibody (AB154335)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PELO antibody (ab154335) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-PELO antibody (AB154335)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PELO antibody (ab154335) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-PELO antibody (AB154335)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PELO antibody (ab154335) at 1/1000 dilution

Lane 1:

Neuro2a whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Lane 3:

Raw264.7 whole cell extracts at 30 µg

Lane 4:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PELO aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9BRX2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PELO also known as Pelota Homolog is a protein with a molecular mass of approximately 44 kDa. It acts as a ribosome-associated protein involved in no-go decay (NGD) a quality control mechanism that resolves stalled ribosomes. PELO helps target and degrade mRNA whose translation is impaired. You can find expression of PELO in various human tissues with predominant activity observed in the heart liver and skeletal muscle.

Biological function summary

PELO contributes to the maintenance of cellular homeostasis by ensuring proper protein synthesis. It acts as part of a surveillance complex that detects and rectifies errors in mRNA translation. This function associates PELO with other factors like HBS1L which help facilitate ribosomal recycling under stress or erroneous conditions. This modulatory role is critical in mitigating potentially harmful effects of translation errors on cellular function.

Pathways

PELO operates within the major ribosome quality control pathway focusing on detecting stalled ribosomes and reclaiming their components for future use. This pathway links PELO with proteins such as HBS1L and ABCE1. These interactions highlight the fine regulatory mechanisms that exist to preserve fidelity and efficiency of translation processes in cells.

PELO exhibits connections with genetic translation-related abnormalities and neurodegenerative disorders. Abnormalities in PELO function correlate with diseases like Pelizaeus-Merzbacher disease which impacts myelin formation in the central nervous system. The connection of PELO with other proteins involved in translation fidelity highlights its potential influence in disorders related to protein synthesis errors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed : 21448132, PubMed : 23667253, PubMed : 27543824, PubMed : 27863242). In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed : 27543824, PubMed : 27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132, PubMed : 32006463). As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy (PubMed : 29861391).

See full target information PELO

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomarker research 12:55 PubMed38831319

2024

Single-cell and spatial transcriptomics reveal alterations in trophoblasts at invasion sites and disturbed myometrial immune microenvironment in placenta accreta spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyuan Ji,Yunshan Chen,Xiuyu Pan,Lina Chen,Xiaodi Wang,Bolun Wen,Junjie Bao,Junmin Zhong,Zi Lv,Zheng Zheng,Huishu Liu

Nucleic acids research 52:5928-5949 PubMed38412259

2024

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ju Tseng,Amy Krans,Indranil Malik,Xiexiong Deng,Evrim Yildirim,Sinem Ovunc,Elizabeth M H Tank,Karen Jansen-West,Ross Kaufhold,Nicolas B Gomez,Roger Sher,Leonard Petrucelli,Sami J Barmada,Peter K Todd

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PELO antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody (AB154335)

Immunocytochemistry/ Immunofluorescence - Anti-PELO antibody (AB154335)

Western blot - Anti-PELO antibody (AB154335)

Western blot - Anti-PELO antibody (AB154335)

Western blot - Anti-PELO antibody (AB154335)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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