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Rabbit Recombinant Monoclonal PELO antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody [EP7530] (AB140615), expandable thumbnail
  • Western blot - Anti-PELO antibody [EP7530] (AB140615), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Not recommended
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed:21448132, PubMed:23667253, PubMed:27543824, PubMed:27863242). In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed:27543824, PubMed:27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed:21448132, PubMed:32006463). As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy (PubMed:29861391).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal PELO antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP7530

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PELO also known as Pelota Homolog is a protein with a molecular mass of approximately 44 kDa. It acts as a ribosome-associated protein involved in no-go decay (NGD) a quality control mechanism that resolves stalled ribosomes. PELO helps target and degrade mRNA whose translation is impaired. You can find expression of PELO in various human tissues with predominant activity observed in the heart liver and skeletal muscle.

Biological function summary

PELO contributes to the maintenance of cellular homeostasis by ensuring proper protein synthesis. It acts as part of a surveillance complex that detects and rectifies errors in mRNA translation. This function associates PELO with other factors like HBS1L which help facilitate ribosomal recycling under stress or erroneous conditions. This modulatory role is critical in mitigating potentially harmful effects of translation errors on cellular function.

Pathways

PELO operates within the major ribosome quality control pathway focusing on detecting stalled ribosomes and reclaiming their components for future use. This pathway links PELO with proteins such as HBS1L and ABCE1. These interactions highlight the fine regulatory mechanisms that exist to preserve fidelity and efficiency of translation processes in cells.

Associated diseases and disorders

PELO exhibits connections with genetic translation-related abnormalities and neurodegenerative disorders. Abnormalities in PELO function correlate with diseases like Pelizaeus-Merzbacher disease which impacts myelin formation in the central nervous system. The connection of PELO with other proteins involved in translation fidelity highlights its potential influence in disorders related to protein synthesis errors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody [EP7530] (ab140615), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody [EP7530] (ab140615)

    Immunohistochemical analysis of paraffin embedded Human squamous cell cervical carcinoma tissue labelling PELO with ab140615 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PELO antibody [EP7530] (ab140615), expandable thumbnail

    Western blot - Anti-PELO antibody [EP7530] (ab140615)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Primary antibody: incubated for 1h at room temperature.

    Secondary antibody: incubated for 1h at room temperature.

    Exposure time:
    Lane 1: 80 seconds
    Lane 2: 20 seconds
    Lane 3: 180 seconds

    All lanes: Western blot - Anti-PELO antibody [EP7530] (ab140615) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

    Lane 3: A375 (Human malignant melanoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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