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AB317204

Anti-PELO antibody [HL2345]

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Rabbit Monoclonal PELO antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human PELO.

View Alternative Names

CGI-17, PELO, Protein pelota homolog, hPelota, Protein Dom34 homolog

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody [HL2345] (AB317204)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody [HL2345] (AB317204)

ab317204 detects PELO protein by immunohistochemical analysis. Sample : Paraffin-embedded rat tissues. PELO stained by ab317204 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody [HL2345] (AB317204)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELO antibody [HL2345] (AB317204)

ab317204 detects PELO protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. PELO stained by ab317204 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-PELO antibody [HL2345] (AB317204)
  • WB

Supplier Data

Western blot - Anti-PELO antibody [HL2345] (AB317204)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317204 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PELO antibody [HL2345] (ab317204) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-PELO antibody [HL2345] (AB317204)
  • WB

Supplier Data

Western blot - Anti-PELO antibody [HL2345] (AB317204)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317204 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PELO antibody [HL2345] (ab317204) at 1/1000 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-PELO antibody [HL2345] (AB317204)
  • WB

Supplier Data

Western blot - Anti-PELO antibody [HL2345] (AB317204)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317204 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PELO antibody [HL2345] (ab317204) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extract at 30 µg

Lane 2:

NIH-3T3 whole cell extract at 30 µg

Lane 3:

Raw264-7 whole cell extract at 30 µg

Lane 4:

C2C12 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-PELO antibody [HL2345] (AB317204)
  • WB

Supplier Data

Western blot - Anti-PELO antibody [HL2345] (AB317204)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317204 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PELO antibody [HL2345] (ab317204) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2345

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Zebrafish

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PELO.

Q9BRX2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PELO also known as Pelota Homolog is a protein with a molecular mass of approximately 44 kDa. It acts as a ribosome-associated protein involved in no-go decay (NGD) a quality control mechanism that resolves stalled ribosomes. PELO helps target and degrade mRNA whose translation is impaired. You can find expression of PELO in various human tissues with predominant activity observed in the heart liver and skeletal muscle.
Biological function summary

PELO contributes to the maintenance of cellular homeostasis by ensuring proper protein synthesis. It acts as part of a surveillance complex that detects and rectifies errors in mRNA translation. This function associates PELO with other factors like HBS1L which help facilitate ribosomal recycling under stress or erroneous conditions. This modulatory role is critical in mitigating potentially harmful effects of translation errors on cellular function.

Pathways

PELO operates within the major ribosome quality control pathway focusing on detecting stalled ribosomes and reclaiming their components for future use. This pathway links PELO with proteins such as HBS1L and ABCE1. These interactions highlight the fine regulatory mechanisms that exist to preserve fidelity and efficiency of translation processes in cells.

PELO exhibits connections with genetic translation-related abnormalities and neurodegenerative disorders. Abnormalities in PELO function correlate with diseases like Pelizaeus-Merzbacher disease which impacts myelin formation in the central nervous system. The connection of PELO with other proteins involved in translation fidelity highlights its potential influence in disorders related to protein synthesis errors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed : 21448132, PubMed : 23667253, PubMed : 27543824, PubMed : 27863242). In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed : 27543824, PubMed : 27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132, PubMed : 32006463). As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy (PubMed : 29861391).
See full target information PELO
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Product promise

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