AB187653

Anti-PELP1 antibody [EPR15212]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PELP1 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

HMX3, MNAR, PELP1, Modulator of non-genomic activity of estrogen receptor, Transcription factor HMX3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] (AB187653)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] (AB187653)

Immunohistochemical analysis of formalin fixed and paraffin embedded Human breast carcinoma tissue labeling PELP1 with ab187653 at 1/100 dilution counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] (AB187653)

Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue labeling PELP1 with ab187653 at 1/100 dilution counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PELP1 antibody [EPR15212] (AB187653)

All lanes:

Western blot - Anti-PELP1 antibody [EPR15212] (ab187653) at 1/5000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 102 kDa,112 kDa,116 kDa,119 kDa,120 kDa,17 kDa,20 kDa,22 kDa,23 kDa,25 kDa,26 kDa,27 kDa,39 kDa,42 kDa,44 kDa,50 kDa,57 kDa,58 kDa,66 kDa,77 kDa,78 kDa

Observed band size: 78 kDa

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Supplier Data

Western blot - Anti-PELP1 antibody [EPR15212] (AB187653)

All lanes:

Western blot - Anti-PELP1 antibody [EPR15212] (ab187653) at 1/2000 dilution

All lanes:

MDA-MB-435 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15212

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Proline Glutamic acid and Leucine-rich Protein 1 (PELP1) also known as Modulator of Nongenomic Activity of Estrogen Receptor (MNAR) plays a significant role in chromatin remodeling and transcriptional regulation. PELP1 has a molecular mass of approximately 160 kDa. It is broadly expressed in various tissues with particularly high levels in reproductive tissues and the brain. PELP1 participates in the recruitment of histone-modifying enzymes and acts as a scaffolding protein that interacts with several nuclear receptors and transcription factors.

Biological function summary

PELP1 acts within the nucleus and is central to gene expression regulation. It is an integral part of diverse protein complexes that engage in remodeling chromatin and modifying transcriptional activity. PELP1 interacts with estrogen receptors where it modulates both genomic and non-genomic signaling pathways affecting gene expression programs related to cell growth and differentiation. Additionally PELP1 associates with various other hormone receptors and transcription factors assisting in the intricate regulation of cell functions.

Pathways

Cellular growth and differentiation rely on the signaling roles that PELP1 performs. PELP1 is involved in the estrogen signaling pathway where it closely interacts with estrogen receptor alpha (ERα). This interaction links estrogen signaling to the cell cycle machinery and PI3K/AKT pathway influencing cell proliferation and survival. Another pathway in which PELP1 plays a critical role is the MAPK/ERK pathway where it cooperates with Src kinase highlighting its importance in cell signaling networks that regulate growth and differentiation.

PELP1 holds a strong association with hormone-driven cancers particularly breast and prostate cancers. In these contexts PELP1's interaction with estrogen and androgen receptors can dramatically alter tumor progression suggesting its role as an oncogene. Additionally aberrant expression and activity of PELP1 have implications in neurological disorders. In breast cancer PELP1 collaborates with ERα to drive estrogen-mediated growth whereas in prostate cancer it engages with androgen receptor (AR) promoting cell proliferation and resistance to therapy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (PubMed : 14963108). Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. Regulates pre-60S association of the critical remodeling factor MDN1 (PubMed : 21326211). May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K.

See full target information PELP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental medicine 25:205 PubMed40517212

2025

HNF4A promotes tumor progression in gastric cancer by transcriptional activation of KIF2C.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Lu,Jin-Heng Gan,Li-Qiang Zhou,Yi-Wu Yuan,Qi Zhou,Lin Xin

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