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AB154830

Anti-PEN2 antibody [EPR9200]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

1

(3 Reviews)

|

(9 Publications)

Rabbit Recombinant Monoclonal PEN2 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 9 publications.

View Alternative Names

PEN2, MDS033, PSENEN, Gamma-secretase subunit PEN-2, Presenilin enhancer protein 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (AB154830)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (AB154830)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PEN2 with ab154830 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PEN2 antibody [EPR9200] (AB154830)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PEN2 antibody [EPR9200] (AB154830)

Intracellular flow cytometric analysis of permeabilized Neuro-2a cells labeling PEN2 with ab154830 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-PEN2 antibody [EPR9200] (AB154830)
  • WB

Lab

Western blot - Anti-PEN2 antibody [EPR9200] (AB154830)

False colour image of Western blot : Anti-PEN2 antibody [EPR9200] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab154830 was shown to bind specifically to PEN2. A band was observed at 12 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in PSENEN knockout cell line ab273720 (knockout cell lysate ab273772). To generate this image, wild-type and PSENEN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 cell lysate at 20 µg

Lane 2:

PSENEN knockout HEK-293 cell lysate at 20 µg

Lane 2:

Western blot - Human PSENEN (PEN2) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-psenen-pen2-knockout-hek-293-cell-line-ab273720'>ab273720</a>)

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Western blot - Anti-PEN2 antibody [EPR9200] (AB154830)
  • WB

Unknown

Western blot - Anti-PEN2 antibody [EPR9200] (AB154830)

All lanes:

Western blot - Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution

Lane 1:

Neuro-2a cell lysate at 10 µg

Lane 2:

Fetal lung tissue lysate at 10 µg

Lane 3:

Human placenta lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 12 kDa

false

  • Carrier free

    Anti-PEN2 antibody [EPR9200] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9200

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (AB76083)

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Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (AB133448)

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEN2 also known as Presenilin Enhancer 2 is a component of the gamma-secretase complex. It has a molecular mass of approximately 12 kDa. PEN2 is expressed in a variety of tissues including neuronal cells and across several cell lines like the HEK 293. This protein plays an essential mechanical role in stabilizing and activating the gamma-secretase complex which is necessary for its protease activity.
Biological function summary

PEN2 is an important element of the gamma-secretase complex composed of presenilin nicastrin APH-1 and PEN2. Its role involves the facilitation of proper maturation and assembly of the complex contributing to the proteolytic processing of several type I transmembrane proteins including the amyloid precursor protein (APP) and Notch. PEN2 plays a part in these cellular processes by ensuring that the gamma-secretase complex functions correctly impacting cellular signaling and communication.

Pathways

Gamma-secretase activity where PEN2 is involved is central to the Notch signaling pathway and the amyloidogenic pathway. The Notch signaling pathway is fundamental in cell differentiation proliferation and apoptosis and relies significantly on the function of the gamma-secretase complex. The amyloidogenic pathway involves the processing of APP to produce amyloid-beta peptides which PEN2's role in the gamma-secretase complex influences. Related proteins in these pathways include presenilin which is an active component in Notch and APP cleavage.

Mutations or dysregulation of PEN2 can have links to Alzheimer's disease and certain cancers. Alzheimer's disease is associated with the production of amyloid-beta peptides which accumulate into plaques a process influenced by PEN2 through gamma-secretase activity with APP. Dysregulated Notch signaling where PEN2 is involved is linked to various cancers due to its role in controlling cell fate decisions. PEN2's interactions with proteins like presenilin and APP highlight its potential impact on these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 12522139, PubMed : 12679784, PubMed : 12740439, PubMed : 12763021, PubMed : 24941111, PubMed : 30598546, PubMed : 30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity (PubMed : 12522139, PubMed : 12679784, PubMed : 12740439, PubMed : 12763021, PubMed : 24941111).
See full target information PSENEN
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature 643:201-209 PubMed39695235

2024

Lithocholic acid binds TULP3 to activate sirtuins and AMPK to slow down ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Qu,Yan Chen,Yu Wang,Weiche Wang,Shating Long,Heng-Ye Yang,Jianfeng Wu,Mengqi Li,Xiao Tian,Xiaoyan Wei,Yan-Hui Liu,Shengrong Xu,Jinye Xiong,Chunyan Yang,Zhenhua Wu,Xi Huang,Changchuan Xie,Yaying Wu,Zheni Xu,Cixiong Zhang,Baoding Zhang,Jin-Wei Feng,Junjie Chen,Yuanji Feng,Huapan Fang,Liyun Lin,Z K Xie,Beibei Sun,Huayu Tian,Yong Yu,Hai-Long Piao,Xiao-Song Xie,Xianming Deng,Chen-Song Zhang,Sheng-Cai Lin

Physiological reports 12:e70157 PubMed39667820

2024

Trim13-induced ubiquitination of RPS27A inhibits the progression of lung cancer by depending on the inactivation of NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lailing Li,Hui Zhou,Yayun Cui,Ke Xu

Nature communications 14:7030 PubMed37919281

2023

A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Liviu Aron,Chenxi Qiu,Zhen Kai Ngian,Marianna Liang,Derek Drake,Jaejoon Choi,Marty A Fernandez,Perle Roche,Emma L Bunting,Ella K Lacey,Sara E Hamplova,Monlan Yuan,Michael S Wolfe,David A Bennett,Eunjung A Lee,Bruce A Yankner

Frontiers in pharmacology 14:1148611 PubMed37144221

2023

Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Ren,Yu-Wei Ding,Lu-Lu Wang,Jian-Dong Jiang

Nature 603:159-165 PubMed35197629

2022

Low-dose metformin targets the lysosomal AMPK pathway through PEN2.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Ma,Xiao Tian,Baoding Zhang,Mengqi Li,Yu Wang,Chunyan Yang,Jianfeng Wu,Xiaoyan Wei,Qi Qu,Yaxin Yu,Shating Long,Jin-Wei Feng,Chun Li,Cixiong Zhang,Changchuan Xie,Yaying Wu,Zheni Xu,Junjie Chen,Yong Yu,Xi Huang,Ying He,Luming Yao,Lei Zhang,Mingxia Zhu,Wen Wang,Zhi-Chao Wang,Mingliang Zhang,Yuqian Bao,Weiping Jia,Shu-Yong Lin,Zhiyun Ye,Hai-Long Piao,Xianming Deng,Chen-Song Zhang,Sheng-Cai Lin

Cell discovery 2:16026 PubMed27625790

2016

Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to γ-secretase cleavage and the Aβ42/Aβ40 ratio.

Applications

WB

Species

Human

Ting-Hai Xu,Yan Yan,Yanyong Kang,Yi Jiang,Karsten Melcher,H Eric Xu

Molecular neurodegeneration 10:39 PubMed26296997

2015

The topology of pen-2, a γ-secretase subunit, revisited: evidence for a reentrant loop and a single pass transmembrane domain.

Applications

Unspecified application

Species

Unspecified reactive species

Xulun Zhang,Chunjiang J Yu,Sangram S Sisodia

Biochemistry 53:4393-406 PubMed24941111

2014

Pen-2 is essential for γ-secretase complex stability and trafficking but partially dispensable for endoproteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Holmes,Swetha Paturi,Dennis J Selkoe,Michael S Wolfe

Journal of neurochemistry 131:94-100 PubMed24865334

2014

Functional analysis and purification of a Pen-2 fusion protein for γ-secretase structural studies.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Holmes,Swetha Paturi,Michael S Wolfe,Dennis J Selkoe
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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