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Rabbit Recombinant Monoclonal PEN2 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 7 publications.


Images

Western blot - Anti-PEN2 antibody [EPR9200] (AB154830), expandable thumbnail
  • Western blot - Anti-PEN2 antibody [EPR9200] (AB154830), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (AB154830), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PEN2 antibody [EPR9200] (AB154830), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIPFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Expected
Mouse
Tested
Expected
Not recommended
Tested
Rat
Predicted
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/500
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:12522139, PubMed:12679784, PubMed:12740439, PubMed:12763021, PubMed:24941111, PubMed:30598546, PubMed:30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity (PubMed:12522139, PubMed:12679784, PubMed:12740439, PubMed:12763021, PubMed:24941111).

Alternative names

PEN2, MDS033, PSENEN, Gamma-secretase subunit PEN-2, Presenilin enhancer protein 2

Recommended products

Rabbit Recombinant Monoclonal PEN2 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9200
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PEN2 also known as Presenilin Enhancer 2 is a component of the gamma-secretase complex. It has a molecular mass of approximately 12 kDa. PEN2 is expressed in a variety of tissues including neuronal cells and across several cell lines like the HEK 293. This protein plays an essential mechanical role in stabilizing and activating the gamma-secretase complex which is necessary for its protease activity.

Biological function summary

PEN2 is an important element of the gamma-secretase complex composed of presenilin nicastrin APH-1 and PEN2. Its role involves the facilitation of proper maturation and assembly of the complex contributing to the proteolytic processing of several type I transmembrane proteins including the amyloid precursor protein (APP) and Notch. PEN2 plays a part in these cellular processes by ensuring that the gamma-secretase complex functions correctly impacting cellular signaling and communication.

Pathways

Gamma-secretase activity where PEN2 is involved is central to the Notch signaling pathway and the amyloidogenic pathway. The Notch signaling pathway is fundamental in cell differentiation proliferation and apoptosis and relies significantly on the function of the gamma-secretase complex. The amyloidogenic pathway involves the processing of APP to produce amyloid-beta peptides which PEN2's role in the gamma-secretase complex influences. Related proteins in these pathways include presenilin which is an active component in Notch and APP cleavage.

Associated diseases and disorders

Mutations or dysregulation of PEN2 can have links to Alzheimer's disease and certain cancers. Alzheimer's disease is associated with the production of amyloid-beta peptides which accumulate into plaques a process influenced by PEN2 through gamma-secretase activity with APP. Dysregulated Notch signaling where PEN2 is involved is linked to various cancers due to its role in controlling cell fate decisions. PEN2's interactions with proteins like presenilin and APP highlight its potential impact on these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PEN2 antibody [EPR9200] (ab154830), expandable thumbnail

    Western blot - Anti-PEN2 antibody [EPR9200] (ab154830)

    False colour image of Western blot: Anti-PEN2 antibody [EPR9200] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab154830 was shown to bind specifically to PEN2. A band was observed at 12 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in PSENEN knockout cell line Human PSENEN (PEN2) knockout HEK-293 cell line ab273720 (knockout cell lysate Human PSENEN (PEN2) knockout HEK-293 cell lysate ab273772). To generate this image, wild-type and PSENEN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: PSENEN knockout HEK-293 cell lysate at 20 µg

    Lane 2: Western blot - Human PSENEN (PEN2) knockout HEK-293 cell line (Human PSENEN (PEN2) knockout HEK-293 cell line ab273720)

    Lane 3: Neuro-2a cell lysate at 20 µg

    Lane 4: Daudi cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Western blot - Anti-PEN2 antibody [EPR9200] (ab154830), expandable thumbnail

    Western blot - Anti-PEN2 antibody [EPR9200] (ab154830)

    All lanes: Western blot - Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution

    Lane 1: Neuro-2a cell lysate at 10 µg

    Lane 2: Fetal lung tissue lysate at 10 µg

    Lane 3: Human placenta lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 12 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (ab154830), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (ab154830)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PEN2 with ab154830 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PEN2 antibody [EPR9200] (ab154830), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PEN2 antibody [EPR9200] (ab154830)

    Intracellular flow cytometric analysis of permeabilized Neuro-2a cells labeling PEN2 with ab154830 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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