Anti-PEN2 antibody [EPR9200] - BSA and Azide free

• WB

Unknown

Western blot - Anti-PEN2 antibody [EPR9200] - BSA and Azide free (AB249136)

This data was developed using ab154830, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PEN2 antibody [EPR9200] (<a href='/en-us/products/primary-antibodies/pen2-antibody-epr9200-ab154830'>ab154830</a>) at 1/1000 dilution

Lane 1:

Neuro-2a cell lysate at 10 µg

Lane 2:

Fetal lung tissue lysate at 10 µg

Lane 3:

Human placenta lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 12 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] - BSA and Azide free (AB249136)

This data was developed using ab154830, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PEN2 with ab154830 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-PEN2 antibody [EPR9200] - BSA and Azide free (AB249136)

False colour image of Western blot : Anti-PEN2 antibody [EPR9200] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab154830 was shown to bind specifically to PEN2. A band was observed at 12 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in PSENEN knockout cell line ab273720 (knockout cell lysate ab273772). To generate this image, wild-type and PSENEN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-PEN2 antibody [EPR9200] (<a href='/en-us/products/primary-antibodies/pen2-antibody-epr9200-ab154830'>ab154830</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 cell lysate at 20 µg

Lane 2:

PSENEN knockout HEK-293 cell lysate at 20 µg

Lane 2:

Western blot - Human PSENEN (PEN2) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-psenen-pen2-knockout-hek-293-cell-line-ab273720'>ab273720</a>)

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 12 kDa

Observed band size: 12 kDa

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