AB226866

Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Pentraxin 3/PTX3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TNFAIP5, TSG14, PTX3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TNF alpha-induced protein 5, TSG-14

3 Images

Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).

-ve control : PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

Supplier Data

Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : HUVEC whole cell lysate 10 μg (Input).
Lane 2 : ab190838 IP in HUVEC whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID : 17389238).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

All lanes:

Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (<a href='/en-us/products/primary-antibodies/pentraxin-3-ptx3-antibody-epr18678-105-ab190838'>ab190838</a>)

Predicted band size: 41 kDa

Observed band size: 42 kDa,47 kDa

true

Carrier free

Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (Capture)
Unconjugated

Anti-Pentraxin 3/PTX3 antibody [EPR18678-105]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18678-105

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

ab226866 is the carrier-free version of ab190838.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pentraxin 3 (PTX3) also known as TSG-14 or TNF-Inducible Gene 14 Protein is an important component of the immune system. Mechanically PTX3 functions as part of the body's defense mechanism against pathogens and tissue damage. It has a molecular weight of approximately 42 kDa and is expressed in a range of tissues such as the immune cells endothelium and epithelial cells. Upon inflammatory signals these cells secrete PTX3 which quickly identifies microbial molecules and apoptotic cells.

Biological function summary

The role of PTX3 extends beyond simple pathogen recognition. It is involved in the regulation of inflammation and tissue homeostasis. As part of the pentraxin superfamily PTX3 plays a role in the formation of complexes with other molecules such as complement components. These interactions modulate the complement cascade an essential part of the innate immune response and influence the resolution of inflammation. PTX3 also interacts with extracellular matrix components stabilizing tissue structure during immune response.

Pathways

PTX3 integrates into vital immune signaling networks. It actively participates in the complement pathway where it regulates the activity of C1q and C3 key complement proteins. Additionally PTX3 connects with the Toll-like receptor (TLR) signaling pathway. This interaction helps modulate the body's response to microbial infections by influencing the activity of TLR4 among other related proteins which are fundamental in recognizing pathogen-associated molecular patterns.

PTX3 is linked to various inflammatory conditions. High levels of PTX3 often indicate a response to cardiovascular diseases such as atherosclerosis. In this context its interactions with C1q highlight its role in modulating disease progression. PTX3 also relates to autoimmune diseases like rheumatoid arthritis where its increased expression correlates with disease severity. In rheumatoid arthritis PTX3 can interact with pro-inflammatory cytokines like TNF-alpha exacerbating the inflammatory process.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

See full target information PTX3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:20 PubMed38989296

2024

Utility of immunocytochemistry in diagnosing abdominopelvic washings from patients undergoing radical surgery for endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Lan,Jing Zhang,Fang Yang,Xin Ma,Rongfang He

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (AB226866)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise