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AB323177

Anti-Pentraxin 3/PTX3 antibody [EPR22094-19]

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Rabbit Recombinant Monoclonal Pentraxin 3/PTX3 antibody. Suitable for I-ELISA, ICC/IF and reacts with Recombinant full length protein - Mouse, Mouse samples.

View Alternative Names

Tsg14, Ptx3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor-inducible gene 14 protein, TSG-14

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Pentraxin 3/PTX3 with ab323177 at 1/2000 (0.258 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing increased cytoplasmic staining in 3T3-L1 cell line (shown in green) treated with Brefeldin A (300 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Pentraxin 3/PTX3 with ab323177 at 1/2000 (0.258 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing increased cytoplasmic staining in NIH/3T3 cell line (shown in green) treated with Brefeldin A (300 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Pentraxin 3/PTX3 with ab323177 at 1/2000 (0.258 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in 3T3-L1 cell line (shown in green) treated with Brefeldin A (300 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : Neuro-2a treated with Brefeldin A (300 ng/mL) for 24 hr.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 (2.5ug/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Pentraxin 3/PTX3 with ab323177 at 1/2000 (0.258 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in green) treated with Brefeldin A (300 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : B16-F10 treated with Brefeldin A (300 ng/mL) for 24 hr.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Indirect ELISA - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] (AB323177)

Indirect ELISA analysis of ab323177 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Mouse Pentraxin 3/PTX3.

Antigen concentration : 1000 ng/ml

  • Carrier free

    Anti-Pentraxin 3/PTX3 antibody [EPR22094-19] - BSA and Azide free (Capture)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22094-19

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein - Mouse": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "125 ng/mL", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
See full target information Ptx3
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Complementary Products

Assay Kits

AB214570

Human Pentraxin 3 / PTX3 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com