AB125007

Anti-Pentraxin 3/PTX3 antibody [EPR6699]

Name: Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007) | Abcam
Brand: Abcam Limited
SKU: AB125007
Price: 5285 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(16 Publications)

Rabbit Recombinant Monoclonal Pentraxin 3/PTX3 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 16 publications.

View Alternative Names

TNFAIP5, TSG14, PTX3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TNF alpha-induced protein 5, TSG-14

4 Images

Supplier Data

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007) at 1/10000 dilution

All lanes:

HUVEC cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Unknown

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007) at 1/1000 dilution

Lane 1:

HL-60 treated with Dexamethasone lysate at 10 µg

Lane 2:

HL-60 lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Lane 5:

HUVEC lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 42 kDa

false

Supplier Data

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007) at 1/10000 dilution

All lanes:

NIH/3T3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

OI-RD Scanning - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Pentraxin 3/PTX3 antibody [EPR6699] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6699

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10 - 1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10 - 1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pentraxin 3 (PTX3) also known as TSG-14 or TNF-Inducible Gene 14 Protein is an important component of the immune system. Mechanically PTX3 functions as part of the body's defense mechanism against pathogens and tissue damage. It has a molecular weight of approximately 42 kDa and is expressed in a range of tissues such as the immune cells endothelium and epithelial cells. Upon inflammatory signals these cells secrete PTX3 which quickly identifies microbial molecules and apoptotic cells.

Biological function summary

The role of PTX3 extends beyond simple pathogen recognition. It is involved in the regulation of inflammation and tissue homeostasis. As part of the pentraxin superfamily PTX3 plays a role in the formation of complexes with other molecules such as complement components. These interactions modulate the complement cascade an essential part of the innate immune response and influence the resolution of inflammation. PTX3 also interacts with extracellular matrix components stabilizing tissue structure during immune response.

Pathways

PTX3 integrates into vital immune signaling networks. It actively participates in the complement pathway where it regulates the activity of C1q and C3 key complement proteins. Additionally PTX3 connects with the Toll-like receptor (TLR) signaling pathway. This interaction helps modulate the body's response to microbial infections by influencing the activity of TLR4 among other related proteins which are fundamental in recognizing pathogen-associated molecular patterns.

PTX3 is linked to various inflammatory conditions. High levels of PTX3 often indicate a response to cardiovascular diseases such as atherosclerosis. In this context its interactions with C1q highlight its role in modulating disease progression. PTX3 also relates to autoimmune diseases like rheumatoid arthritis where its increased expression correlates with disease severity. In rheumatoid arthritis PTX3 can interact with pro-inflammatory cytokines like TNF-alpha exacerbating the inflammatory process.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

See full target information PTX3

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1468579 PubMed39584137

2024

Celecoxib and rofecoxib have different effects on small intestinal ischemia/reperfusion injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Szilvia B László,Barbara Hutka,András S Tóth,Tamás Hegyes,Zsuzsanna O Demeter,Arezoo Haghighi,Gerda Wachtl,Ágnes Kelemen,Anna Jakab,Klára Gyires,Zoltán S Zádori

Open life sciences 18:20220735 PubMed37941784

2023

PTX3 promotes IVIG resistance-induced endothelial injury in Kawasaki disease by regulating the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Sun,Lihua Liu,Ruihua Yang

Autophagy 19:1997-2014 PubMed36647288

2023

PRMT1 driven PTX3 regulates ferritinophagy in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Kirti Lathoria,Pruthvi Gowda,Sonia B Umdor,Shruti Patrick,Vaishali Suri,Ellora Sen

Aging 14:4036-4049 PubMed35522573

2022

Pentraxin 3 depletion (PTX3 KD) inhibited myocardial fibrosis in heart failure after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Yufang Xu,Yiting Hu,Yanping Geng,Na Zhao,Caiyun Jia,Haojing Song,Wanjun Bai,Caihui Guo,Lili Wang,Yanhui Ni,Xiaoyong Qi

Stem cells (Dayton, Ohio) 39:581-599 PubMed33547700

2021

SMAD-specific E3 ubiquitin ligase 2 promotes angiogenesis by facilitating PTX3 degradation in MSCs from patients with ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjun Ma,Wen Yang,Zhaopeng Cai,Peng Wang,Hongyu Li,Rujia Mi,Yuhang Jiang,Zhongyu Xie,Pengfei Sui,Yanfeng Wu,Huiyong Shen

Cytotechnology 73:91-100 PubMed33505117

2021

MiR-29b-3p aggravates cardiac hypoxia/reoxygenation injury via targeting PTX3.

Applications

Unspecified application

Species

Unspecified reactive species

Dan He,Lei Yan

Journal of biomaterials applications 35:592-601 PubMed32873118

2020

Amniotic membrane allografts maintain key biological properties post SCCO and lyophilization processing.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer S McDaniel,Jennifer L Wehmeyer,Lauren E Cornell,Anthony J Johnson,David O Zamora

The Journal of neuroscience : the official journal 40:7355-7374 PubMed32817249

2020

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yujiao Lu,Gangadhara R Sareddy,Jing Wang,Quanguang Zhang,Fu-Lei Tang,Uday P Pratap,Rajeshwar R Tekmal,Ratna K Vadlamudi,Darrell W Brann

Antioxidants (Basel, Switzerland) 9: PubMed32012711

2020

Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects.

Applications

Unspecified application

Species

Unspecified reactive species

Brisamar Estébanez,Alexandra L Rodriguez,Nishant P Visavadiya,Michael Whitehurst,María J Cuevas,Javier González-Gallego,Chun-Jung Huang

Journal of autoimmunity 106:102336 PubMed31601476

2019

Complement C1q synergizes with PTX3 in promoting NLRP3 inflammasome over-activation and pyroptosis in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xun-Yao Wu,Ke-Tian Li,Hua-Xia Yang,Bo Yang,Xin Lu,Li-Dan Zhao,Yun-Yun Fei,Hua Chen,Li Wang,Jing Li,Ling-Yi Peng,Wen-Jie Zheng,Yong Hou,Ying Jiang,Qun Shi,Wen Zhang,Feng-Chun Zhang,Jian-Min Zhang,Bo Huang,Wei He,Xuan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pentraxin 3/PTX3 antibody [EPR6699]

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

OI-RD Scanning - Anti-Pentraxin 3/PTX3 antibody [EPR6699] (AB125007)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise