Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487)

This data was developed using ab90806, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution. Positive staining on mouse placenta was observed. Nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487)

This data was developed using ab90806, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen rat placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution. Positive staining on rat placenta was observed. Nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• WB

Lab

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487)

This data was developed using ab90806, the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1, 2 : 15 seconds; Lane 3 : 3 seconds; Lane 4 : 180 seconds.

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487) at 1/1000 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Mouse placenta tissue lysate at 20 µg

Lane 3:

Rat placenta tissue lysate at 20 µg

Lane 4:

3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 41 kDa

false

• WB

Lab

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487)

This data was developed using ab90806, the same antibody clone in a different buffer formulation.

The expression profile observed is consistent with what has been described in the literature (PMID : 17389238).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487) at 1/1000 dilution

Lane 1:

Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 41 kDa

false

Exposure time: 15s