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Rat Recombinant Monoclonal Pentraxin 3/PTX3 antibody. Carrier free. Suitable for WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487), expandable thumbnail
  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (AB264487), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rat
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBIHC-Fr
Human
Not recommended
Tested
Expected
Mouse
Not recommended
Tested
Tested
Rat
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

Alternative names

TNFAIP5, TSG14, PTX3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TNF alpha-induced protein 5, TSG-14

Recommended products

Rat Recombinant Monoclonal Pentraxin 3/PTX3 antibody. Carrier free. Suitable for WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
MNB1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab264487 is the carrier-free version of Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pentraxin 3 (PTX3) also known as TSG-14 or TNF-Inducible Gene 14 Protein is an important component of the immune system. Mechanically PTX3 functions as part of the body's defense mechanism against pathogens and tissue damage. It has a molecular weight of approximately 42 kDa and is expressed in a range of tissues such as the immune cells endothelium and epithelial cells. Upon inflammatory signals these cells secrete PTX3 which quickly identifies microbial molecules and apoptotic cells.

Biological function summary

The role of PTX3 extends beyond simple pathogen recognition. It is involved in the regulation of inflammation and tissue homeostasis. As part of the pentraxin superfamily PTX3 plays a role in the formation of complexes with other molecules such as complement components. These interactions modulate the complement cascade an essential part of the innate immune response and influence the resolution of inflammation. PTX3 also interacts with extracellular matrix components stabilizing tissue structure during immune response.

Pathways

PTX3 integrates into vital immune signaling networks. It actively participates in the complement pathway where it regulates the activity of C1q and C3 key complement proteins. Additionally PTX3 connects with the Toll-like receptor (TLR) signaling pathway. This interaction helps modulate the body's response to microbial infections by influencing the activity of TLR4 among other related proteins which are fundamental in recognizing pathogen-associated molecular patterns.

Associated diseases and disorders

PTX3 is linked to various inflammatory conditions. High levels of PTX3 often indicate a response to cardiovascular diseases such as atherosclerosis. In this context its interactions with C1q highlight its role in modulating disease progression. PTX3 also relates to autoimmune diseases like rheumatoid arthritis where its increased expression correlates with disease severity. In rheumatoid arthritis PTX3 can interact with pro-inflammatory cytokines like TNF-alpha exacerbating the inflammatory process.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487), expandable thumbnail

    Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

    This data was developed using Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806, the same antibody clone in a different buffer formulation.

    The expression profile observed is consistent with what has been described in the literature (PMID:17389238).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487) at 1/1000 dilution

    Lane 1: Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 2: HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution

    Predicted band size: 41 kDa

    Exposure time: 15s

  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

    This data was developed using Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse placenta tissue labeling Pentraxin 3/PTX3 with Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806 at 1/20 dilution followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on mouse placenta was observed. Nuclear counterstain is DAPI (Blue).
    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487), expandable thumbnail

    Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

    This data was developed using Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 15 seconds; Lane 3: 3 seconds; Lane 4: 180 seconds.

    All lanes: Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487) at 1/1000 dilution

    Lane 1: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: Mouse placenta tissue lysate at 20 µg

    Lane 3: Rat placenta tissue lysate at 20 µg

    Lane 4: 3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution

    Predicted band size: 41 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

    This data was developed using Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen rat placenta tissue labeling Pentraxin 3/PTX3 with Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806 at 1/20 dilution followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on rat placenta was observed. Nuclear counterstain is DAPI (Blue).
    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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