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AB34793

Anti-PEP Carboxylase antibody

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(7 Publications)

Rabbit Polyclonal CAPP1 antibody. Suitable for IP, ELISA, WB and reacts with Corn samples. Cited in 7 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to PEP Carboxylase.

View Alternative Names

PPC, PEP1, Phosphoenolpyruvate carboxylase 1, PEPC 1, PEPCase 1

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Corn

Applications

ELISA, WB, IP

applications

Specificity

Cross reactivity against Phospho-enol-pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Corn": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/30000 - 1/160000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" } } }

Product details

Antibody concentration was determined by Refractometry.

Properties and storage information

Form
Liquid
Purity
Whole antiserum
Purification notes
ab34793 was prepared from monospecific antiserum by a delipidation and defibrination.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEP Carboxylase also known as phosphoenolpyruvate carboxylase or PEP-carboxylase is an enzyme that catalyzes the conversion of phosphoenolpyruvate (PEP) to oxaloacetate. This enzyme is important for fixing carbon in photosynthetic pathways. The enzyme's molecular mass is approximately 100 kDa and it primarily expresses in plant tissues particularly those involved in photosynthesis such as mesophyll cells. It also appears in some bacteria where it plays a role in carbon metabolism.
Biological function summary

Phosphoenolpyruvate carboxylase plays a significant role in the fixation of carbon dioxide contributing to C4 and CAM photosynthesis mechanisms where it takes part in the assimilation of inorganic carbon into organic compounds. It functions as a homotetramer where each subunit contributes to overall enzyme activity. This enzyme not only aids in photosynthesis but also participates in processes like gluconeogenesis and anaplerotic reactions in non-photosynthetic organisms highlighting its diverse roles across species.

Pathways

Phosphoenolpyruvate carboxylase's role is integral to both the C4 and CAM photosynthetic pathways. In these pathways it works alongside other proteins such as Rubisco to mitigate photorespiration and increase photosynthetic efficiency especially under conditions of drought or high temperatures. This enzyme also intersects with the glycolysis and gluconeogenesis pathways connecting to intermediary metabolism through shared molecules like phosphoenolpyruvate and oxaloacetate linking carbohydrate metabolism to broader metabolic networks.

Phosphoenolpyruvate carboxylase may relate to metabolic disorders due to its central role in gluconeogenesis and anaplerosis. Dysregulation of this enzyme's activity can potentially impact conditions such as diabetes where gluconeogenesis plays a pivotal role in glucose production. Moreover certain microbes engineered with this enzyme hold potential in bioenergy applications and may influence pathogenicity in bacterial infections linking PEP-carboxylase function indirectly to infectious disease dynamics. Its interactions with proteins involved in metabolism suggests a broader impact that goes beyond plant biology possibly affecting human health through metabolic balances.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
See full target information PEP1

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 60: PubMed35244192

2022

LncCCAT1 interaction protein PKM2 upregulates SREBP2 phosphorylation to promote osteosarcoma tumorigenesis by enhancing the Warburg effect and lipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Pu,Jianxiang Liu,Doudou Jing,Fengxia Chen,Xin Huang,Deyao Shi,Wei Wu,Hui Lin,Lei Zhao,Zhenhao Zhang,Xiao Lv,Baichuan Wang,Zhicai Zhang,Zengwu Shao

Plant communications 2:100215 PubMed34327325

2021

Paladin, a tyrosine phosphatase-like protein, is required for -mediated immunity in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Tsung-Chi Chen,Mawsheng Chern,Michael Steinwand,Deling Ruan,Yu Wang,Arkin Isharani,Pamela Ronald

Nature communications 10:3252 PubMed31324801

2019

TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yongliang Zhang,Gaoyuan Song,Neeraj K Lal,Ugrappa Nagalakshmi,Yuanyuan Li,Wenjie Zheng,Pin-Jui Huang,Tess C Branon,Alice Y Ting,Justin W Walley,Savithramma P Dinesh-Kumar

Cell host & microbe 23:485-497.e5 PubMed29649442

2018

The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Neeraj K Lal,Ugrappa Nagalakshmi,Nicholas K Hurlburt,Rosalva Flores,Aurelie Bak,Pyae Sone,Xiyu Ma,Gaoyuan Song,Justin Walley,Libo Shan,Ping He,Clare Casteel,Andrew J Fisher,Savithramma P Dinesh-Kumar

Developmental cell 44:348-361.e7 PubMed29290588

2018

Arabidopsis ARGONAUTE 1 Binds Chromatin to Promote Gene Transcription in Response to Hormones and Stresses.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Ying Xin,Le Xu,Zhaokui Cai,Yuanchao Xue,Yong Liu,Daoxin Xie,Yule Liu,Yijun Qi

PloS one 10:e0122936 PubMed25860807

2015

The dynamic of the splicing of bZIP60 and the proteins encoded by the spliced and unspliced mRNAs reveals some unique features during the activation of UPR in Arabidopsis thaliana.

Applications

Unspecified application

Species

Arabidopsis thaliana

Juan Parra-Rojas,Adrian A Moreno,Irina Mitina,Ariel Orellana

PloS one 6:e28469 PubMed22145048

2011

The metabolic reprogramming evoked by nitrosative stress triggers the anaerobic utilization of citrate in Pseudomonas fluorescens.

Applications

sELISA

Species

Pseudomonas fluorescens

Christopher Auger,Joseph Lemire,Dominic Cecchini,Adam Bignucolo,Vasu D Appanna
View all publications

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