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AB113690

Anti-PEP1 antibody [18C8BC7AD10]

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(3 Publications)

Mouse Monoclonal PEP1 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications.

View Alternative Names

VPS10, VPT1, YBL017C, YBL03.22, YBL0302, PEP1, Vacuolar protein sorting/targeting protein VPS10, Carboxypeptidase Y receptor, Carboxypeptidase Y-deficient protein 1, Sortilin VPS10, Vacuolar carboxypeptidase sorting receptor VPS10, Vacuolar protein sorting-associated protein 10, Vacuolar protein-targeting protein 1, CPY receptor, VPS10, VPT1, YBL017C, YBL03.22, YBL0302, PEP1, Vacuolar protein sorting/targeting protein VPS10, Carboxypeptidase Y receptor, Carboxypeptidase Y-deficient protein 1, Sortilin VPS10, Vacuolar carboxypeptidase sorting receptor VPS10, Vacuolar protein sorting-associated protein 10, Vacuolar protein-targeting protein 1, CPY receptor

1 Images
Western blot - Anti-PEP1 antibody [18C8BC7AD10] (AB113690)
  • WB

Unknown

Western blot - Anti-PEP1 antibody [18C8BC7AD10] (AB113690)

All lanes:

Western blot - Anti-PEP1 antibody [18C8BC7AD10] (ab113690)

All lanes:

Yeast whole cell lysate at 15 µg

Predicted band size: 178 kDa

true

  • Unconjugated

    Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control

  • Carrier free

    Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

18C8BC7AD10

Isotype

IgG2c

Light chain type

kappa

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEP1 also known as peptide 1 is an enzyme with a molecular mass of approximately 50 kDa. It catalyzes the hydrolysis of peptide bonds which is essential for protein metabolism. PEP1 shows expression in several tissues with high levels detected in the liver and kidneys. The enzyme is responsible for breaking down specific peptide substrates contributing to the regulation of peptide levels within cells.
Biological function summary

PEP1 helps regulate neuropeptide activity and peptide hormone degradation processes. It does not appear to be part of a larger protein complex but its activity still balances peptide turnover in the body. Its enzyme action ensures that peptides do not accumulate to potentially harmful levels therefore keeping cellular environments stable.

Pathways

PEP1 participates in key metabolic and signaling pathways such as the neuropeptide signaling pathway and the renin-angiotensin system. In these pathways PEP1 interacts with related proteins such as angiotensin-converting enzyme (ACE) to help control blood pressure and maintain homeostasis. Its functional role in these pathways situates PEP1 as a mediator of diverse physiological processes.

PEP1 has connections to conditions such as hypertension and metabolic syndrome. Dysfunction or altered activity of PEP1 can affect peptide metabolism influencing blood pressure regulation and metabolic balance. The enzyme's association with ACE implies that alterations in PEP1 activity may contribute to or exacerbate these conditions. Understanding its function could provide insights into therapeutic strategies for managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.
See full target information PEP1

Additional targets

PEP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2049:165-190 PubMed31602611

2019

Mapping the Saccharomyces cerevisiae Spatial Proteome with High Resolution Using hyperLOPIT.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J H Nightingale,Stephen G Oliver,Kathryn S Lilley

Bio-protocol 9:e3303 PubMed33654815

2019

A Protocol to Map the Spatial Proteome Using HyperLOPIT in .

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J H Nightingale,Kathryn S Lilley,Stephen G Oliver

Frontiers in molecular neuroscience 10:207 PubMed28713240

2017

The Coordinated Action of Calcineurin and Cathepsin D Protects Against α-Synuclein Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Aufschnaiter,Lukas Habernig,Verena Kohler,Jutta Diessl,Didac Carmona-Gutierrez,Tobias Eisenberg,Walter Keller,Sabrina Büttner
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Product promise

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