JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB113689

Anti-PEP12 antibody [2C3G4]

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse Monoclonal PEP12 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications.

View Alternative Names

VPS6, VPT13, YOR036W, OR26.29, PEP12, Syntaxin PEP12, Carboxypeptidase Y-deficient protein 12, Vacuolar protein sorting-associated protein 6, Vacuolar protein-targeting protein 13

1 Images
Western blot - Anti-PEP12 antibody [2C3G4] (AB113689)
  • WB

Unknown

Western blot - Anti-PEP12 antibody [2C3G4] (AB113689)

All lanes:

Western blot - Anti-PEP12 antibody [2C3G4] (ab113689)

All lanes:

Yeast whole cell lysate at 15 µg

Predicted band size: 33 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2C3G4

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEP12 also known as Vps45-dependent receptor or Vps45 is a protein that plays a mechanical role in vesicle trafficking. It is involved in the fusion of vesicles with their target membranes participating in the transport of proteins and lipids within cells. The protein with a molecular mass of approximately 12 kDa is expressed in yeast and other eukaryotic organisms. It primarily localizes to membranes of the Golgi apparatus and vacuoles where it participates in membrane fusion events necessary for intracellular transport.
Biological function summary

PEP12 contributes to the selective docking and fusion of vesicles. As a part of the SNARE complex an important structure for vesicle fusion PEP12 aids in tethering docking and the eventual fusion of vesicles and their target membranes. It works alongside proteins such as Vps45 and other SNARE components coordinating vesicular trafficking in cellular compartments. This role ensures efficient sorting and proper distribution of lipids and proteins essential for cell function and homeostasis.

Pathways

PEP12 functions in the secretory and endocytic pathways. In the secretory pathway it ensures the delivery of cargo from the trans-Golgi network to the vacuole or lysosome. In the endocytic pathway PEP12 is involved in recycling molecules back to the Golgi or in their delivery to other cellular destinations. The protein interacts closely with other SNARE proteins and factors like Sec1p family members which regulate its activity and ensure precise targeting of vesicles during these processes.

PEP12's role in vesicle trafficking links it to various conditions characterized by faulty protein and lipid transport. Research has indicated a connection between defects in components of the SNARE complex including PEP12 and neurodegenerative disorders such as Alzheimer's disease. The protein also interfaces with Rab proteins which are central to vesicle budding movement and fusion. Errors in vesicular trafficking could result in accumulation of cellular waste contributing to disease pathogenesis highlighting the need to understand PEP12's functions for potential therapeutic targeting.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in the sorting and targeting of vacuolar proteases.
See full target information PEP12

Additional targets

PEP12
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Autophagy reports 4:2511724 PubMed40458443

2025

A plasmid module for PCR-based gene modification for the accurate measurement of vacuolar delivery of specific proteins in yeast .

Applications

Unspecified application

Species

Unspecified reactive species

Jakob Valdbjørn Kanne,Fulvio Reggiori

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

STAR protocols 4:101935 PubMed36520633

2022

Protocol to purify and detect ubiquitinated phospholipids in budding yeast and human cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ichi Sakamaki,Noboru Mizushima
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB113688

Anti-PHO8 antibody [1D3A10]

Western blot - Anti-PHO8 antibody [1D3A10] (AB113688)

  • 0
  • 0
Primary Antibodies

AB22676

Anti-Rpt5/TBP-1 antibody

Western blot - Anti-Rpt5/TBP-1 antibody (AB22676)

  • 0
  • 0
Primary Antibodies

AB315472

Anti-GCN1 antibody [EPR28542-86]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-GCN1 antibody [EPR28542-86] (AB315472)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com