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AB50123

Anti-Pepsinogen I antibody [7G3]

4

(3 Reviews)

|

(4 Publications)

Anti-Pepsinogen I antibody [7G3] (ab50123) is a mouse monoclonal antibody detecting Pepsinogen I in IHC-P. Suitable for Human.

- Trusted since 2007

View Alternative Names

Pepsinogen, Pepsinogen-1, Pepsinogen-A

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (AB50123)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (AB50123)

IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7G3

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-Pepsinogen I antibody [7G3] (ab50123) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pepsinogen I often referred to simply as pepsinogen is a zymogen form of the digestive enzyme pepsin. It possesses a molecular mass of approximately 44 kDa. This inactive precursor is synthesized and secreted by the gastric chief cells located in the stomach lining. Once released into the gastric lumen pepsinogen undergoes conversion into the active enzyme pepsin when exposed to the acidic pH of the stomach which facilitates protein breakdown during digestion.
Biological function summary

Pepsinogen I serves as an important component in the proteolytic process within the digestive system. It is not a part of a larger protein complex but functions independently after its activation. The conversion to pepsin enables the digestion of dietary proteins into smaller peptides and amino acids which are essential for the absorption and utilization by the body. This activity is fundamental to maintaining efficient nutrient processing and absorption in the gastrointestinal tract.

Pathways

The digestive process involving pepsinogen I is closely tied to the gastric acid secretion pathway. The enzyme pepsin derived from pepsinogen plays an important role in this pathway by breaking down proteins within the acidic environment of the stomach. Additionally pepsinogen activation indirectly interacts with other enzymes like gastric lipase which aids in the digestion of fats highlighting the interconnectedness of digestive pathways that ensure the body efficiently utilizes nutrients.

Pepsinogen I levels may be associated with conditions like gastritis and peptic ulcer disease. Aberrant expression or secretion can serve as a marker for these gastric disorders where pepsinogen I through its converted form pepsin can be involved in the excessive breakdown of the stomach lining. Additionally its relationship with gastric-related proteins like mucins which protect against acidic damage further accentuates its significance in maintaining gastric health and the potential implications when balance is disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Pepsinogen I
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 10:3053-3058 PubMed35116613

2022

A case report of endoscopic submucosal dissection for a new subtype of gastric adenoma: mixed fundic and pyloric mucosa type.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Han,Yuyong Tan,Deliang Liu,Songqing Fan,Lei Shi,Chengbai Liang

Nature protocols 16:3802-3835 PubMed34215862

2021

CODEX multiplexed tissue imaging with DNA-conjugated antibodies.

Applications

mIHC

Species

Human

Sarah Black,Darci Phillips,John W Hickey,Julia Kennedy-Darling,Vishal G Venkataraaman,Nikolay Samusik,Yury Goltsev,Christian M Schürch,Garry P Nolan

Journal of oncology 2020:5701527 PubMed32411238

2020

Cell Survival Is Regulated via SOX9/BCL2L1 Axis in HCT-116 Colorectal Cancer Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Lizárraga-Verdugo,Erika Ruiz-García,César López-Camarillo,Mercedes Bermúdez,Mariana Avendaño-Félix,Rosalío Ramos-Payán,Geovanni Romero-Quintana,Alfredo Ayala-Ham,Carlos Villegas-Mercado,Carlos Pérez-Plasencia,Maribel Aguilar-Medina

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 30:171-88 PubMed20038200

2009

Identification of cellular genes induced in human cells after activation of the OAS/RNaseL pathway by vaccinia virus recombinants expressing these antiviral enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Domingo-Gil,José Manuel González,Mariano Esteban
View all publications
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