Rabbit Recombinant Monoclonal Pepsinogen II/PGC antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse, Rat, Human, Recombinant fragment - Human samples. Cited in 1 publication.
Images
![Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--western-blot-img53192.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826)")
![Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--western-blot-img8490.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826)")
![Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--western-blot-img20568.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92958.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112079.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (AB255826)")
Publications
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- Gastroenterology 163:411-425.e42022Toll-like Receptor 9 Pathway Mediates Schlafen-MDSC Polarization During Helicobacter-induced Gastric Metaplasias.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Ding,Jayati Chakrabarti,Sulaiman Sheriff,Qian Li,Hahn Nguyen Thi Hong,Ricky A Sontz,Zoe E Mendoza,Amanda Schreibeis,Michael A Helmrath,Yana Zavros,Juanita L MerchantPubMed 35487288
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Expected |
| Mouse | Tested | Not recommended | Tested | Tested |
| Rat | Tested | Not recommended | Tested | Tested |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Alternative names
Gastricsin, Pepsinogen C, PGC
Recommended products
Rabbit Recombinant Monoclonal Pepsinogen II/PGC antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse, Rat, Human, Recombinant fragment - Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22843-269
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pepsinogen II also known as PGC or progastricsin functions as a digestive zymogen activated to pepsin in the acidic environment of the stomach. This enzyme precursor has a molecular mass of approximately 42-47 kDa. It is primarily expressed in the gastric chief cells and mucous cells of the stomach lining. Pepsinogen II plays an important role in the initial digestion of proteins breaking down complex protein molecules into peptides.
- Biological function summary
Pepsinogen II contributes to digestive processes by converting into active pepsin at low pH levels facilitating protein degradation. Unlike some other digestive enzymes pepsin works independently from a complex. The production and secretion of pepsinogen II align with the gastric acid production ensuring efficient digestive activity. Its expression is vital for proper nutritional absorption and maintenance of gastrointestinal health.
- Pathways
Pepsinogen II is closely involved in the proteolytic enzyme pathway. It connects with other gastric enzymes such as pepsin A through the activation processes influenced by gastric acid secretion. This interplay among digestive enzymes ensures optimal protein breakdown and nutrient absorption. The regulatory mechanisms that modulate gastric acid and pepsinogen secretion play an integral role in maintaining the digestive efficiency.
- Associated diseases and disorders
Pepsinogen II levels can serve as biomarkers for certain gastric conditions including gastritis and gastric cancer. Altered levels of pepsinogen II are associated with atrophic gastritis where reduced enzyme levels reflect damage to gastric mucosa. Also increased serum pepsinogen II concentration can indicate potential gastric cancer risk. These conditions highlight pepsinogen II's clinical importance in gastrointestinal disease diagnosis and management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--western-blot-img53192.jpg "Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
Bands of 38KDa and 32KDa represent the precursor and the mature form. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28424202).
Negative control: Human testis and MDA-MB-231 (PMID: 11259249 and 8663058 ).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time: Lane 1:10 seconds; Lanes 2-4:3 minutes.
All lanes: Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826) at 1/1000 dilution
Lane 1: Human stomach lysate at 20 µg
Lane 2: Human testis lysate at 20 µg
Lane 3: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Rat stomach lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lane 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 32 kDa, 38 kDa
![Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--western-blot-img8490.jpg "Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
This blot was developed using a higher sensitivity ECL substrate. Blocking/Diluting buffer and concentration: 5% NFDM/TBST Exposure Time: 37 seconds
All lanes: Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826) at 1/1000 dilution
All lanes: Mouse stomach lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 32 kDa, 38 kDa
![Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--western-blot-img20568.jpg "Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time: 3 minutes.
All lanes: Western blot - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826) at 1/1000 dilution
All lanes: His-tagged Recombinant Human Pepsinogen C/PGC protein(aa1-388), 10ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92958.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat stomach. The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112079.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse stomach. The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113236.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human stomach (PMID 10883891). The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-frozen-sections-img55687.jpg "Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat stomach tissue labeling Annexin-4/ANXA4 with ab255826 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on rat gastric mucosa (PMID: 10883891) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-frozen-sections-img77751.jpg "Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse stomach tissue labeling Annexin-4/ANXA4 with ab255826 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse gastric mucosa (PMID: 10883891) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113236.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
![Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-frozen-sections-img55687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
![Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826), expandable thumbnail](https://content.abcam.com/products/images/pepsinogen-ii-pgc-antibody-epr22843-269-ab255826--immunohistochemistry-frozen-sections-img77751.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] (ab255826)")
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