AB131246

Anti-Peptide YY/PYY antibody

Name: Anti-Peptide YY/PYY antibody (ab131246) | Abcam
Brand: Abcam Limited
SKU: AB131246
Price: 510 USD
Availability: InStock
Rating: 5 (5 reviews)

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What is this?

(5 Reviews)

(3 Publications)

Rabbit Polyclonal Peptide YY/PYY antibody. Suitable for IHC-P and reacts with Rat, Human, Common marmoset samples. Cited in 3 publications.

View Alternative Names

Peptide YY, PYY, PYY-I, Peptide tyrosine tyrosine

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

IHC image of Peptide YY/PYY staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131246, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

AbReview39466****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

IHC image of Peptide YY/PYY staining in Human colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21°C. Sample was incubated in primary antibody at 1/8000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

Image courtesy of Carl Hobbs, Kings College London, U.K.

IHC-P

Collaborator31270****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

IHC image of Peptide YY/PYY staining in mouse pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21°C. Sample was incubated in primary antibody at 1/6000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

Image courtesy of Carl Hobbs, Kings College London, U.K.

IHC-P

AbReview39570****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

IHC image of Peptide YY/PYY staining in marmoset Jejunum formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21°C. Sample was incubated in primary antibody at 1/5000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

Image Courtesy of Carl Hobbs, Kings College London, U.K.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Common marmoset, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peptide YY (PYY) also known as PYY1 or PYY3-36 is a 36-amino acid peptide hormone with a molecular weight of approximately 4 kDa. Expressed primarily in the L-cells of the ileum and colon PYY is released into the bloodstream postprandially. This hormone serves as an important messenger in appetite control and energy homeostasis. Peptide YY reduces gastric motility and increases water and electrolyte absorption in the colon playing an integral role in digestion and nutrient absorption processes.

Biological function summary

The hormone influences several physiological functions beyond its mechanical roles. Peptide YY hormone function includes inhibiting appetite through interactions with the neuropeptide Y (NPY) receptors in the brain's hypothalamus. It acts together with similar peptides including pancreatic polypeptide (PP) and neuropeptide Y (NPY) establishing a complex regulatory system that governs energy balance and food intake. PYY contributes to short-term and long-term body weight management linking gut to brain signals.

Pathways

Peptide YY hormone function integrates into the gut-brain axis and the neuroendocrine system. It engages mainly in the regulation of the Y2 receptor pathway interacting with proteins like the ghrelin receptor and insulin pathways. Moreover PYY can influence the feeding pathway by modulating the arcuate nucleus neurons establishing connections with the melanocortin system and agouti-related peptide (AgRP). This integration importantly contributes to satiety and appetite modulation.

Peptide YY is linked to obesity and irritable bowel syndrome (IBS). Altered levels of PYY associate with increased risk of obesity due to dysregulated appetite signals. Disorders like IBS relate to the imbalance of functional gut hormones including PYY which impacts bowel motility and gastrointestinal comfort. Other proteins such as ghrelin and leptin participate in these pathways providing insight into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.

See full target information PYY

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Archives of pathology & laboratory medicine 147:797-807 PubMed36191342

2022

Clinicopathologic Impact of Peptide Hormonal Expression in Rectal Neuroendocrine Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jisup Kim,Dong-Hoon Yang,HaeSung Jung,HyungJun Cho,Hyeung-Jin Jang,Changhoon Yoo,In Ja Park,Baek-Yeol Ryoo,Jin-Sook Ryu,Seung-Mo Hong

JCI insight 2: PubMed28614797

2017

Neuropeptide Y expression marks partially differentiated β cells in mice and humans.

Applications

ICC/IF

Species

Mouse

Pope Rodnoi,Mohan Rajkumar,Abu Saleh Md Moin,Senta K Georgia,Alexandra E Butler,Sangeeta Dhawan

Tissue & cell 46:535-9 PubMed25458814

2014

Amylin-like immunoreactivity in pancreatic X cells of the black-spotted frog Rana (Pelophylax) nigromaculata.

Applications

IHC-Fr

Species

Frog

Hirohumi Suzuki,Toshiharu Yamamoto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Peptide YY/PYY antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB131246)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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