Anti-Peptide YY/PYY antibody

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB22663)

IHC image of Peptide YY/PYY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21°C. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

Image courtesy of Carl Hobbs, Kings College London, U.K.

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB22663)

IHC image of Peptide YY/PYY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21°C. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

Image courtesy of Carl Hobbs, Kings College London, U.K.

• IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Peptide YY/PYY antibody (AB22663)

Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (AB22663)

ab22663 staining Peptide YY/PYY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue (left, WT; right, Peptide YY/PYY KO) was fixed with paraformaldehyde and blocked with 3% H₂O₂ in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).

Image from Acosta A et al., PLoS One. 2011;6(10):e26137. Epub 2011 Oct 10. Fig 2.; doi:10.1371/journal.pone.0026137; October 10, 2011, PLoS ONE 6(10): e26137.