AB259771

Anti-Peptide YY/PYY antibody [EPR21011-6] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Peptide YY/PYY antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Peptide YY, PYY, PYY-I, Peptide tyrosine tyrosine

1 Images

Sandwich ELISA - Anti-Peptide YY/PYY antibody [EPR21011-6] - BSA and Azide free (Capture) (AB259771)

sELISA

Supplier Data

Sandwich ELISA - Anti-Peptide YY/PYY antibody [EPR21011-6] - BSA and Azide free (Capture) (AB259771)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab255727).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21011-6

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab259771 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PYY. The recommended pair for sandwich ELISA is:
Capture: ab259771, Human PYY Capture Antibody (unconjugated)
Detector: ab259772, Human PYY Detector Antibody (unconjugated)
The reference range value is 15.63 - 1000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peptide YY (PYY) also known as PYY1 or PYY3-36 is a 36-amino acid peptide hormone with a molecular weight of approximately 4 kDa. Expressed primarily in the L-cells of the ileum and colon PYY is released into the bloodstream postprandially. This hormone serves as an important messenger in appetite control and energy homeostasis. Peptide YY reduces gastric motility and increases water and electrolyte absorption in the colon playing an integral role in digestion and nutrient absorption processes.

Biological function summary

The hormone influences several physiological functions beyond its mechanical roles. Peptide YY hormone function includes inhibiting appetite through interactions with the neuropeptide Y (NPY) receptors in the brain's hypothalamus. It acts together with similar peptides including pancreatic polypeptide (PP) and neuropeptide Y (NPY) establishing a complex regulatory system that governs energy balance and food intake. PYY contributes to short-term and long-term body weight management linking gut to brain signals.

Pathways

Peptide YY hormone function integrates into the gut-brain axis and the neuroendocrine system. It engages mainly in the regulation of the Y2 receptor pathway interacting with proteins like the ghrelin receptor and insulin pathways. Moreover PYY can influence the feeding pathway by modulating the arcuate nucleus neurons establishing connections with the melanocortin system and agouti-related peptide (AgRP). This integration importantly contributes to satiety and appetite modulation.

Peptide YY is linked to obesity and irritable bowel syndrome (IBS). Altered levels of PYY associate with increased risk of obesity due to dysregulated appetite signals. Disorders like IBS relate to the imbalance of functional gut hormones including PYY which impacts bowel motility and gastrointestinal comfort. Other proteins such as ghrelin and leptin participate in these pathways providing insight into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.

See full target information PYY

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Peptide YY/PYY antibody [EPR21011-6] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-Peptide YY/PYY antibody [EPR21011-6] - BSA and Azide free (Capture) (AB259771)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise