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Rabbit Polyclonal PER3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PER3 aa 1-150.


Images

Western blot - Anti-PER3 antibody (AB224594), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody (AB224594), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody (AB224594), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PER3 antibody (AB224594), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PER3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P56645

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/500 - 1/5000

Notes

-

Species

Mouse

Dilution info

1/500 - 1/5000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20 - 1/200

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/200

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

Originally described as a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1, NR1D2, RORA, RORB and RORG, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. Has a redundant role with the other PER proteins PER1 and PER2 and is not essential for the circadian rhythms maintenance. In contrast, plays an important role in sleep-wake timing and sleep homeostasis probably through the transcriptional regulation of sleep homeostasis-related genes, without influencing circadian parameters. Can bind heme.

Alternative names

Recommended products

Rabbit Polyclonal PER3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PER3 aa 1-150.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PER3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P56645
Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The PER3 protein also known as Period circadian protein homolog 3 plays an important role in maintaining the circadian rhythm. It has a molecular weight of approximately 126 kDa. PER3 is ubiquitously expressed with higher expression noted in tissues such as liver brain and heart. In particular its expression in the suprachiasmatic nucleus of the brain highlights its role in circadian regulation.

Biological function summary

PER3 contributes to the regulation of circadian rhythms and is a part of the circadian clock complex. Together with PER1 and PER2 PER3 participates in feedback loops that control transcriptional-translational cycles which impact various bodily functions such as sleep and metabolism. It binds with proteins like CRY1 CRY2 CLOCK and BMAL1 to establish and maintain the body's 24-hour cycle.

Pathways

PER3 integrates into the circadian rhythm signaling pathway. It interacts directly with other proteins like CLOCK and BMAL1 to modulate gene expression influencing sleep patterns hormonal control and metabolic processes. These interactions highlight its essential role in maintaining homeostasis within the circadian clock machinery and its impact on the sleep-wake cycle.

Associated diseases and disorders

PER3's dysfunction associates with sleep disorders and certain mood disorders. Variants in the PER3 gene have shown links to delayed sleep phase disorder and bipolar disorder. Additionally PER3's interaction with other proteins such as PER1 and PER2 plays a significant role in these pathways indicating how disturbances in these interactions can lead to circadian-related pathologies.

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4 product images

  • Western blot - Anti-PER3 antibody (ab224594), expandable thumbnail

    Western blot - Anti-PER3 antibody (ab224594)

    All lanes: Western blot - Anti-PER3 antibody (ab224594) at 1/500 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lane 2: Mouse skeletal tissue lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 132 kDa

    Observed band size: 132 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody (ab224594), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody (ab224594)

    Paraffin-embedded human ovarian cancer tissue stained for PER3 using ab224594 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody (ab224594), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody (ab224594)

    Paraffin-embedded human colon cancer tissue stained for PER3 using ab224594 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-PER3 antibody (ab224594), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PER3 antibody (ab224594)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PER3 (green) using ab224594 at 1/100 dilution in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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