AB117496

PerCP Anti-6X His tag® antibody [AD1.1.10]

Name: Anti-6X His tag® PerCP antibody [AD1.1.10] (ab117496) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB117496
Price: 510 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal 6X His tag® antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1.1.10

Isotype

IgG1

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/percp-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab118658'>ab118658</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 95% Sodium phosphate, 1.71% Sucrose, 0.58% Sodium chloride, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:6393-6406 PubMed33995664

2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect .

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhong,Wei Shi,Lu Gan,Xiuli Liu,Yu Huo,Pan Wu,Zhikun Zhang,Tao Wu,Hongmei Peng,Yong Huang,Yongxiang Zhao,Yulin Yuan,Zhiming Deng,Hongliang Tang

Scientific reports 8:5332 PubMed29593284

2018

Cementoin-SLPI fusion protein binds to human monocytes and epithelial cells and shows higher biological activity than SLPI.

Applications

Unspecified application

Species

Unspecified reactive species

Paulo C Maffía,Diego Guerrieri,Ximena Villalonga,Fiorella Caro,Sonia Gómez,Nancy Tateosian,Betiana P Bogado,Mercedes L Sánchez,Nella Ambrosi,Eduardo Chuluyan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP Anti-6X His tag® antibody [AD1.1.10]

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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