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Rabbit Recombinant Monoclonal CD146 antibody - conjugated to PerCP. Suitable for Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - PerCP Anti-CD146 antibody [EPR3208] (AB216361), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species

Human

Dilution info

1/50

Notes

The cellular localisation of this product has been verified in ICC/IF.PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab222107 - Rabbit monoclonal IgG (PerCP), is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

5 products for Alternative Version

Target data

Function

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Alternative names

Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, MCAM, MUC18

Recommended products

Rabbit Recombinant Monoclonal CD146 antibody - conjugated to PerCP. Suitable for Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3208

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry (Intracellular) - PerCP Anti-CD146 antibody [EPR3208] (ab216361), expandable thumbnail

    Flow Cytometry (Intracellular) - PerCP Anti-CD146 antibody [EPR3208] (ab216361)

    Overlay histogram showing A375 cells stained with ab216361 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216361, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP (PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.

    This antibody gave a positive signal in A375 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com