JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB114860

PerCP Anti-CD20 antibody [2H7]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal CD20 antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing MS4A1 protein.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

1 Images
Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (AB114860)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (AB114860)

Surface staining of human peripheral blood with ab114860.

  • 660 APC

    APC Anti-CD20 antibody [2H7]

  • 660 APC

    APC Anti-CD20 antibody [2H7]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD20 antibody [2H7]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD20 antibody [2H7]

  • Biotin

    Biotin Anti-CD20 antibody [2H7]

  • 519 FITC

    FITC Anti-CD20 antibody [2H7]

  • 519 FITC

    FITC Anti-CD20 antibody [2H7]

  • 578 PE

    PE Anti-CD20 antibody [2H7]

  • 578 PE

    PE Anti-CD20 antibody [2H7]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD20 antibody [2H7]

  • 618 PE/DyLight™ 594

    PE/DyLight™ 594 Anti-CD20 antibody [2H7]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD20 antibody [2H7]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2H7

Isotype

IgG2b

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

No

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11836

style
left-column

Alternative Products

Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
Primary Antibodies

AB78237

Anti-CD20 antibody [EP459Y]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD20 antibody [EP459Y] (AB78237)

  • 4
  • 7
Primary Antibodies

AB166865

Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (AB166865)

  • 0
  • 0
Primary Antibodies

AB198941

Alexa Fluor® 488 Anti-CD20 antibody [EP459Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD20 antibody [EP459Y] (AB198941)

  • 4
  • 3
Primary Antibodies

AB198943

Alexa Fluor® 647 Anti-CD20 antibody [EP459Y]

Recombinant|Advanced Validation|RabMAb

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD20 antibody [EP459Y] (AB198943)

  • 4
  • 1
Primary Antibodies

AB236434

Anti-CD20 antibody [SP32] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] - BSA and Azide free (AB236434)

  • 5
  • 3

Complementary Products

Assay Kits

AB285273

Human CD20 ELISA Kit

Sandwich ELISA - Human CD20 ELISA Kit (AB285273)

  • 0
  • 0
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB208514

Alexa Fluor® 555 Anti-CD3D antibody [EP4426]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (AB208514)

  • 4
  • 2
Primary Antibodies

AB201825

Alexa Fluor® 647 Anti-PD1 antibody [EPR4877(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PD1 antibody [EPR4877(2)] (AB201825)

  • 5
  • 1
Primary Antibodies

AB281930

Alexa Fluor® 594 Anti-CD21 antibody [EP3093]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD21 antibody [EP3093] (AB281930)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/en-us/products/primary-antibodies/percp-mouse-igg2b-kappa-monoclonal-mpc-11-isotype-control-ab172890'>ab172890</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/en-us/products/primary-antibodies/percp-mouse-igg2b-kappa-monoclonal-mpc-11-isotype-control-ab172890'>ab172890</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

Purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 273:344-8 PubMed9417086

1998

Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment.

Applications

Unspecified application

Species

Unspecified reactive species

J P Deans,S M Robbins,M J Polyak,J A Savage

The Journal of biological chemistry 270:22632-8 PubMed7545683

1995

Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20.

Applications

Unspecified application

Species

Unspecified reactive species

J P Deans,L Kalt,J A Ledbetter,G L Schieven,J B Bolen,P Johnson
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com