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AB88034

PerCP Anti-CD28 antibody [CD28.2]

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(7 Publications)

Mouse Monoclonal CD28 antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing CD28 protein.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, TP44

1 Images
Flow Cytometry - PerCP Anti-CD28 antibody [CD28.2] (AB88034)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP Anti-CD28 antibody [CD28.2] (AB88034)

Flow cytometry analysis (surface staining) of human peripheral blood cells using ab88034.

  • Carrier free

    Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CD28.2

Isotype

IgG1

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.

P10747

Specificity

Reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

The purified antibody (>95% by SDS-PAGE) is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The conjugate is purified by size-exclusion chromatography andadjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).
See full target information CD28
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 78:6995-7003 PubMed15194776

2004

Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates.

Applications

Unspecified application

Species

Unspecified reactive species

Sook-Hyang Jeong,Ming Qiao,Michelina Nascimbeni,Zongyi Hu,Barbara Rehermann,Krishna Murthy,T Jake Liang

Molecular and cellular biology 24:3860-73 PubMed15082780

2004

Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Scharschmidt,Elmar Wegener,Vigo Heissmeyer,Anjana Rao,Daniel Krappmann

Journal of immunology (Baltimore, Md. : 1950) 166:197-206 PubMed11123293

2000

Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6.

Applications

Unspecified application

Species

Unspecified reactive species

F Marti,A Krause,N H Post,C Lyddane,B Dupont,M Sadelain,P D King

Journal of immunology (Baltimore, Md. : 1950) 163:3511-5 PubMed10477625

1999

Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

A Tazi,J Moreau,A Bergeron,S Dominique,A J Hance,P Soler

Journal of immunology (Baltimore, Md. : 1950) 163:62-70 PubMed10384100

1999

Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

J Galea-Lauri,D Darling,S U Gan,L Krivochtchapov,M Kuiper,J Gäken,B Souberbielle,F Farzaneh

The Biochemical journal 293 ( Pt 3):835-42 PubMed8394695

1993

Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity.

Applications

Unspecified application

Species

Unspecified reactive species

J Nunes,S Klasen,M D Franco,C Lipcey,C Mawas,M Bagnasco,D Olive

International immunology 5:311-5 PubMed8385476

1993

CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events.

Applications

Unspecified application

Species

Unspecified reactive species

J Nunès,S Klasen,M Ragueneau,C Pavon,D Couez,C Mawas,M Bagnasco,D Olive
View all publications
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