AB106215

PerCP Anti-CD3 antibody [UCHT1]

Name: Anti-CD3 PerCP antibody [UCHT1] (ab106215) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB106215
Price: 170 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal CD3d antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images

Flow Cytometry - PerCP Anti-CD3 antibody [UCHT1] (AB106215)

Flow Cyt

Unknown

Flow Cytometry - PerCP Anti-CD3 antibody [UCHT1] (AB106215)

Flow Cytometry analysis of Human peripheral blood cells labeling CD3 with Anti-CD3 antibody [UCHT1], prediluted (PerCP) (ab106215).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHT1

Isotype

IgG1

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

Specificity

ab106215 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. It reacts with the epsilon chain of the CD3 complex. HLDA I; WS Code T 3 HLDA III; WS Code T 126 HLDA III; WS Code T 471 HLDA VI; WS Code T 6T-CD3.1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/percp-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab118658'>ab118658</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/percp-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab118658'>ab118658</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).

See full target information CD3D

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 14:3399-3406 PubMed29042925

2017

Calreticulin is an effective immunologic adjuvant to tumor-associated antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Zhi Peng Gao,Song Qin,Chang Bai Liu,Li Li Zou

The Journal of experimental medicine 204:2537-44 PubMed17923503

2007

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Gabrielle M Siegers,Mahima Swamy,Edgar Fernández-Malavé,Susana Minguet,Sylvia Rathmann,Alberto C Guardo,Verónica Pérez-Flores,Jose R Regueiro,Balbino Alarcón,Paul Fisch,Wolfgang W A Schamel

American journal of respiratory cell and molecular biology 32:177-84 PubMed15576669

2004

Identification and characterization of human pulmonary dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ingel K Demedts,Guy G Brusselle,Karim Y Vermaelen,Romain A Pauwels

Proceedings of the National Academy of Sciences of 101:16268-73 PubMed15534202

2004

Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly L Arnett,Stephen C Harrison,Don C Wiley

Journal of immunology (Baltimore, Md. : 1950) 170:5947-55 PubMed12794121

2003

TCR dynamics in human mature T lymphocytes lacking CD3 gamma.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar S Torres,Andrés Alcover,David A Zapata,Jacques Arnaud,Alberto Pacheco,José M Martín-Fernández,Eugenia M Villasevil,Ozden Sanal,José R Regueiro

Journal of immunology (Baltimore, Md. : 1950) 161:1113-22 PubMed9686569

1998

Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

S le Gouvello,V Manceau,A Sobel

Nature 298:375-7 PubMed6178042

1982

Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.

Applications

Unspecified application

Species

Unspecified reactive species

J A Garson,P C Beverley,H B Coakham,E I Harper

View all publications

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