AB65952

PerCP Anti-CD45 antibody [MEM-28]

Name: PerCP Anti-CD45 antibody [MEM-28] (ab65952) | Abcam
Brand: Abcam Limited
SKU: AB65952
Price: 170 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal CD45 antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

2 Images

Flow Cytometry - PerCP Anti-CD45 antibody [MEM-28] (AB65952)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP Anti-CD45 antibody [MEM-28] (AB65952)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab65952 (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - PerCP Anti-CD45 antibody [MEM-28] (AB65952)

Separation of human lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab65952 (10 μl reagent / 100 μl of peripheral whole blood).

Unconjugated

Anti-CD45 antibody [MEM-28]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD45 antibody [MEM-28]
660 APC

APC Anti-CD45 antibody [MEM-28]
Biotin

Biotin Anti-CD45 antibody [MEM-28]
778 PE/Cy7®

PE/Cy7® Anti-CD45 antibody [MEM-28]
785 APC/Cy7®

APC/Cy7® Anti-CD45 antibody [MEM-28]
618 PE/DyLight™ 594

PE/DyLight™ 594 Anti-CD45 antibody [MEM-28]
519 FITC

FITC Anti-CD45 antibody [MEM-28]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD45 antibody [MEM-28]
578 PE

PE Anti-CD45 antibody [MEM-28]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-28

Isotype

IgG1

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Specificity

ab65952 reacts with all alternative forms of human CD45 antigen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.<br><br><a href='/en-us/products/primary-antibodies/percp-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab118658'>ab118658</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

purity >95% by PAGE-PAGE

Storage buffer

Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Bosnian journal of basic medical sciences 19:43-51 PubMed29688163

2019

Human amniotic fluid stem cells (hAFSCs) expressing p21 and cyclin D1 genes retain excellent viability after freezing with (dimethyl sulfoxide) DMSO.

Applications

Unspecified application

Species

Unspecified reactive species

Shiva Gholizadeh-Ghaleh Aziz,Zahra Fardyazar,Fatima Pashaei-Asl,Mohammad Rahmati-Yamchi,Khodadad Khodadadi,Maryam Pashaiasl

Molecular medicine reports 18:920-930 PubMed29845202

2018

Regulatory effects of miRNA‑181a on FasL expression in bone marrow mesenchymal stem cells and its effect on CD4+T lymphocyte apoptosis in estrogen deficiency‑induced osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyi Shao,Xiaohui Fu,Yang Yu,Deqin Yang

PloS one 11:e0158281 PubMed27434028

2016

Isolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells.

Applications

Flow Cyt

Species

Human

Shiva Gholizadeh-Ghaleh Aziz,Fatima Pashaei-Asl,Zahra Fardyazar,Maryam Pashaiasl

Journal of immunology (Baltimore, Md. : 1950) 186:6543-52 PubMed21525379

2011

Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.

Applications

Flow Cyt

Species

Human

Salima Sadallah,Ceylan Eken,Perrine J Martin,Jürg A Schifferli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP Anti-CD45 antibody [MEM-28]

Flow Cytometry - PerCP Anti-CD45 antibody [MEM-28] (AB65952)

Flow Cytometry - PerCP Anti-CD45 antibody [MEM-28] (AB65952)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise