AB77227

PerCP Anti-CD63 antibody [MEM-259]

Name: PerCP Anti-CD63 antibody [MEM-259] (ab77227) | Abcam
Brand: Abcam Limited
SKU: AB77227
Price: 170 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(3 Publications)

Mouse Monoclonal CD63 antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD63 protein.

View Alternative Names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

1 Images

Flow Cytometry - PerCP Anti-CD63 antibody [MEM-259] (AB77227)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP Anti-CD63 antibody [MEM-259] (AB77227)

Flow cytometry analysis of IgE-activated peripheral blood stained using ab77227.

Unconjugated

Anti-CD63 antibody [MEM-259]
578 PE

PE Anti-CD63 antibody [MEM-259]
667 PE/Cy5®

PE/Cy5® Anti-CD63 antibody [MEM-259]
Biotin

Biotin Anti-CD63 antibody [MEM-259]
519 FITC

FITC Anti-CD63 antibody [MEM-259]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-259

Isotype

IgG1

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information.

P08962

Specificity

ab77227 reacts with CD63 expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p><p><a href='/en-us/products/primary-antibodies/percp-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab118658'>ab118658</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

The antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

See full target information CD63

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Lab on a chip 19:3326-3336 PubMed31497821

2019

Size-based analysis of extracellular vesicles using sequential transfer of an evaporating droplet.

Applications

Unspecified application

Species

Unspecified reactive species

Hwapyeong Jeong,Hyunwoo Shin,Johan Yi,Yonghyun Park,Jiyoul Lee,Yogesh Gianchandani,Jaesung Park

Scientific reports 6:21961 PubMed26911789

2016

Bone marrow stromal/stem cell-derived extracellular vesicles regulate osteoblast activity and differentiation in vitro and promote bone regeneration in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhao Qin,Lian Wang,Zhengliang Gao,Genyin Chen,Changqing Zhang

EMBO reports 5:883-8 PubMed15332114

2004

The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Cerny,Yan Feng,Anan Yu,Katsuya Miyake,Barbara Borgonovo,Judith Klumperman,Jacopo Meldolesi,Paul L McNeil,Tomas Kirchhausen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP Anti-CD63 antibody [MEM-259]

Flow Cytometry - PerCP Anti-CD63 antibody [MEM-259] (AB77227)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise