JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB220509

PerCP Anti-CD68 antibody [FA-11]

Be the first to review this product! Submit a review

|

(2 Publications)

Rat Monoclonal CD68 antibody - conjugated to PerCP. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD68, Macrosialin, Cd68

1 Images
Flow Cytometry (Intracellular) - PerCP Anti-CD68 antibody [FA-11] (AB220509)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PerCP Anti-CD68 antibody [FA-11] (AB220509)

Overlay histogram showing Raw264.7 cells stained with ab220509 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220509, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.

  • 660 APC

    APC Anti-CD68 antibody [FA-11]

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-CD68 antibody [FA-11]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD68 antibody [FA-11]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD68 antibody [FA-11]

  • HRP

    HRP Anti-CD68 antibody [FA-11]

  • 578 PE

    PE Anti-CD68 antibody [FA-11]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

FA-11

Isotype

IgG2a

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB213363

Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] (AB213363)

  • 4
  • 6
Primary Antibodies

AB222914

Alexa Fluor® 488 Anti-CD68 antibody [KP1]

Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD68 antibody [KP1] (AB222914)

  • 4
  • 2
Primary Antibodies

AB224029

Alexa Fluor® 647 Anti-CD68 antibody [EPR20545]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] (AB224029)

  • 0
  • 0
Primary Antibodies

AB227458

Anti-CD68 antibody [EPR20545] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458)

  • 4
  • 1
Primary Antibodies

AB277938

Alexa Fluor® 594 Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD68 antibody [EPR20545] (AB277938)

  • 0
  • 0
Primary Antibodies

AB53444

Anti-CD68 antibody [FA-11]

Lab Essentials|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

  • 4
  • 25

Complementary Products

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB204467

Alexa Fluor® 647 Anti-F4/80 antibody [F4/80]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-F4/80 antibody [F4/80] (AB204467)

  • 0
  • 0
Primary Antibodies

AB209027

Alexa Fluor® 647 Anti-iNOS antibody [EPR16635]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-iNOS antibody [EPR16635] (AB209027)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information Cd68
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:14924-14939 PubMed40290979

2025

Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Vinícius Barreto Garcia,Luiz H S Gasparotto,Aurigena A de Araujo,Renata F C Leitão,Gerly A C Brito,Natalia Feitosa Vilar,Emily Lima Oliveira,Paulo M M Guedes,Raimundo F de Araújo Júnior

Frontiers in bioengineering and biotechnology 9:756755 PubMed34746108

2021

Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ibrahim Fathi,Takehiro Imura,Akiko Inagaki,Yasuhiro Nakamura,Ayman Nabawi,Masafumi Goto
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com