AB82410

PerCP Anti-CD80 antibody [MEM-233]

Name: Anti-CD80 PerCP antibody [MEM-233] (ab82410) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB82410
Price: 170 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD80 antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD80.

View Alternative Names

CD80, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7

1 Images

Flow Cytometry - PerCP Anti-CD80 antibody [MEM-233] (AB82410)

Flow Cyt

Unknown

Flow Cytometry - PerCP Anti-CD80 antibody [MEM-233] (AB82410)

Flow Cytometry analysis of P815 cells labeling CD80 with Anti-CD80 antibody [MEM-233] (PerCP) (ab82410).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-233

Isotype

IgG1

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD80. The exact immunogen used to generate this antibody is proprietary information.

P33681

Specificity

ab82410 reacts with CD80 expressed on professional antigen presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100 μl of whole blood.</p><p><a href='/en-us/products/primary-antibodies/percp-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab118658'>ab118658</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Conjugate made from highly purified antibody (>95% by SDS-PAGE).

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.

Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed : 10438913). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed : 12121659). Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed : 36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

See full target information CD80

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 91:364-8 PubMed16531259

2006

Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment.

Applications

Flow Cyt

Species

Human

Diana Campioni,Sabrina Moretti,Luisa Ferrari,Marina Punturieri,Gian Luigi Castoldi,Francesco Lanza

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP Anti-CD80 antibody [MEM-233]

Flow Cytometry - PerCP Anti-CD80 antibody [MEM-233] (AB82410)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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