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AB77131

PerCP Anti-CD86 antibody [BU63]

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(2 Publications)

Mouse Monoclonal CD86 antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD86 protein.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

1 Images
Flow Cytometry - PerCP Anti-CD86 antibody [BU63] (AB77131)
  • Flow Cyt

Unknown

Flow Cytometry - PerCP Anti-CD86 antibody [BU63] (AB77131)

ab77131 staining, surface CD86 in human peripheral blood cells by Flow Cytometery. Monocytes were used for gating. The blue peak reperesents negative control while red peak show staining with ab77131.

  • Carrier free

    Anti-CD86 antibody [BU63] - BSA and Azide free

  • Unconjugated

    Anti-CD86 antibody [BU63]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD86 antibody [BU63]

  • 519 FITC

    FITC Anti-CD86 antibody [BU63]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD86 antibody [BU63]

  • 660 APC

    APC Anti-CD86 antibody [BU63]

  • 578 PE

    PE Anti-CD86 antibody [BU63]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BU63

Isotype

IgG1

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD86 protein. The exact immunogen used to generate this antibody is proprietary information.

P42081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/percp-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab118658'>ab118658</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
ab77131 is purified by size exclusion chromatography and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed : 12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 40:1563-1576 PubMed34727384

2021

Mg -mediated autophagy-dependent polarization of macrophages mediates the osteogenesis of bone marrow stromal stem cells by interfering with macrophage-derived exosomes containing miR-381.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhu,Shushan Zhao,Liang Cheng,Zhangyuan Lin,Min Zeng,Zhe Ruan,Buhua Sun,Zhongwei Luo,Yifu Tang,Haitao Long

Acta pharmaceutica Sinica. B 9:794-808 PubMed31384539

2019

Development of the triazole-fused pyrimidine derivatives as highly potent and reversible inhibitors of histone lysine specific demethylase 1 (LSD1/KDM1A).

Applications

Unspecified application

Species

Unspecified reactive species

Zhonghua Li,Lina Ding,Zhongrui Li,Zhizheng Wang,Fengzhi Suo,Dandan Shen,Taoqian Zhao,Xudong Sun,Junwei Wang,Ying Liu,Liying Ma,Bing Zhao,Pengfei Geng,Bin Yu,Yichao Zheng,Hongmin Liu
View all publications
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