AB216354

PerCP Anti-CD90 / Thy1 antibody [MRC OX-7]

Lab Essentials
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(1 Publication)

Mouse Monoclonal CD90 / Thy1 antibody - conjugated to PerCP. Suitable for Flow Cyt (Intra) and reacts with Rat samples. Cited in 1 publication.

View Alternative Names

CD90, Thy-1, Thy1, Thy-1 membrane glycoprotein, Thy-1 antigen

1 Images

Flow Cytometry (Intracellular) - PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (AB216354)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (AB216354)

Flow cytometry overlay histogram showing PC12 (NGF differentiated) cells stained with ab216354 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab216354) (1x 106 in 100μl at 5μg/ml (1/100)) for 30 min at 22°C. Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50mW Blue laser (488nm) and 690/50 bandpass filter.

519 FITC

FITC Anti-CD90 / Thy1 antibody [MRC OX-7]
HRP

HRP Anti-CD90 / Thy1 antibody [MRC OX-7]
578 PE

PE Anti-CD90 / Thy1 antibody [MRC OX-7]
Unconjugated

Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker
Carrier free

Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRC OX-7

Isotype

IgG1

Light chain type

kappa

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

Reacts with

Rat

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" }, "Horse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information Thy1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:931 PubMed39719443

2024

Mechanistic insights into SIRT7 and EZH2 regulation of cisplatin resistance in bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yudong Cao,Shuo Wang,Jinchao Ma,Mengping Long,Xiuli Ma,Xiao Yang,Yongpeng Ji,Xingxing Tang,Jia Liu,Chen Lin,Yong Yang,Peng Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP Anti-CD90 / Thy1 antibody [MRC OX-7]

Flow Cytometry (Intracellular) - PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (AB216354)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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