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Rat Monoclonal ITAL antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Mouse samples.

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Images

Flow Cytometry - PerCP/Cy5.5® Anti-CD11a antibody [M17/4] (AB246417), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Rat

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

0.25 μg

Associated Products

Select an associated product type

1 product for Alternative Version

4 products for Alternative Product

Target data

Function

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4 (PubMed:2051027). Integrin ITGAL/ITGB2 is a receptor for F11R (By similarity). Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:16234355, PubMed:24158516). Acts as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (By similarity). Required for generation of common lymphoid progenitor cells in bone marrow, indicating the role in lymphopoiesis (PubMed:25108025). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.

Alternative names

Recommended products

Rat Monoclonal ITAL antibody - conjugated to PerCP. Suitable for Flow Cyt and reacts with Mouse samples.

Key facts

Isotype

IgG2a

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Form

Liquid

Clonality

Monoclonal

Clone number

M17/4

Purification technique

Affinity purification

Light chain type

kappa

Concentration
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Purification notes

ab246417 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD11a also known as ITGAL and LFA-1 alpha chain or integrin alpha L is a protein that functions as an important component of the immune system. It has a molecular mass of approximately 180 kDa. CD11a is expressed predominantly in leukocytes enabling these immune cells to adhere and interact with other cells. This adhesive property is especially critical in the trafficking of leukocytes to sites of inflammation and infection. CD11a pairs with CD18 to form the leukocyte function-associated antigen-1 (LFA-1) complex which plays a significant role in cell signaling and immune responses.

Biological function summary

This protein enables leukocyte adhesion and transendothelial migration which are essential for the immune surveillance process. CD11a is a part of the integrin family known for regulating cell adhesion and interactions with the extracellular matrix. By forming a heterodimer with CD18 it mediates tight adhesion between immune cells and endothelial cells. This interaction aids in the immune response by enhancing the activation and movement of T cells and other leukocytes through the vascular endothelium into tissues.

Pathways

The protein participates in the immune cell trafficking pathway and the integrin signaling pathway. In these pathways CD11a contributes to the modulation of immune responses and cell movement. The interaction of CD11a with intercellular adhesion molecule 1 (ICAM-1) and other proteins within these pathways emphasizes its role in the firm adhesion of leukocytes during inflammation and infection ultimately facilitating leukocyte extravasation and tissue infiltration.

Associated diseases and disorders

CD11a is highly relevant to autoimmune diseases such as psoriasis and multiple sclerosis. For instance in psoriasis the overactive immune response involves overexpression of integrins including CD11a leading to inflammation. The protein Efalizumab a monoclonal antibody against CD11a has been used in the treatment of psoriasis to reduce leukocyte adhesion and activation. Such relationships highlight CD11a's critical role in both normal immune function and pathological conditions.

Product promise

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1 product image

  • Flow Cytometry - PerCP/Cy5.5® Anti-CD11a antibody [M17/4] (ab246417), expandable thumbnail

    Flow Cytometry - PerCP/Cy5.5® Anti-CD11a antibody [M17/4] (ab246417)

    C57Bl/6 splenocytes were stained with 0.25 μg ab246417 (Solid blue line) or 0.25 μg PerCP-Cyanine5.5 Rat IgG2a isotype control (Dashed line).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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