AB157312

PerCP/Cy5.5® Anti-CD14 antibody [MEM-15]

Name: Anti-CD14 PerCP/Cy5.5® antibody [MEM-15] (ab157312) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB157312
Price: 225 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal CD14 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

2 Images

Flow Cytometry - PerCP/Cy5.5® Anti-CD14 antibody [MEM-15] (AB157312)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD14 antibody [MEM-15] (AB157312)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab157312 (4 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD14 antibody [MEM-15] (AB157312)

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab157312 (4 µl reagent / 100 µl of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-15

Isotype

IgG1

Light chain type

kappa

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human CD14.

P08571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>4 μl reagent / 100 μl of whole blood. <a href='/en-us/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>4 μl reagent / 100 μl of whole blood. <a href='/en-us/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Purified antibody is conjugated with tandem dye PerCP/Cy5.5^® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biochemical engineering journal 150: PubMed32831621

2019

Effect of Monocyte Seeding Density on Dendritic Cell Generation in an Automated Perfusion-Based Culture System.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Kozbial,Lekhana Bhandary,Shashi K Murthy

Journal of immunological methods 457:53-65 PubMed29625078

2018

Automated generation of immature dendritic cells in a single-use system.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Kozbial,Lekhana Bhandary,Bradley B Collier,Christopher S Eickhoff,Daniel F Hoft,Shashi K Murthy

The Journal of experimental medicine 196:1017-24 PubMed12391013

2002

Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Sing,Dagmar Rost,Natalia Tvardovskaia,Andreas Roggenkamp,Agnès Wiedemann,Carsten J Kirschning,Martin Aepfelbacher,Jürgen Heesemann

Infection and immunity 69:3772-81 PubMed11349042

2001

CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited.

Applications

Unspecified application

Species

Unspecified reactive species

D P Funda,L Tucková,M A Farré,T Iwase,I Moro,H Tlaskalová-Hogenová

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP/Cy5.5® Anti-CD14 antibody [MEM-15]

Flow Cytometry - PerCP/Cy5.5® Anti-CD14 antibody [MEM-15] (AB157312)

Flow Cytometry - PerCP/Cy5.5® Anti-CD14 antibody [MEM-15] (AB157312)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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