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AB157321

PerCP/Cy5.5® Anti-CD20 antibody [2H7]

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(1 Publication)

Mouse Monoclonal CD20 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MS4A1 protein.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

3 Images
Flow Cytometry - PerCP/Cy5.5® Anti-CD20 antibody [2H7] (AB157321)
  • Flow Cyt

Unknown

Flow Cytometry - PerCP/Cy5.5® Anti-CD20 antibody [2H7] (AB157321)

Flow Cytometry analysis of human peripheral blood cells labeling CD20 with Anti-CD20 antibody [2H7] (PerCP/Cy5.5®) (ab157321).

Flow Cytometry - PerCP/Cy5.5® Anti-CD20 antibody [2H7] (AB157321)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD20 antibody [2H7] (AB157321)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab157321 (concentration in sample 1.67 µg/ml).

Flow Cytometry - PerCP/Cy5.5® Anti-CD20 antibody [2H7] (AB157321)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD20 antibody [2H7] (AB157321)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab157321 (concentration in sample 1.67 µg/ml).

  • 660 APC

    APC Anti-CD20 antibody [2H7]

  • 660 APC

    APC Anti-CD20 antibody [2H7]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD20 antibody [2H7]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD20 antibody [2H7]

  • Biotin

    Biotin Anti-CD20 antibody [2H7]

  • 519 FITC

    FITC Anti-CD20 antibody [2H7]

  • 519 FITC

    FITC Anti-CD20 antibody [2H7]

  • 578 PE

    PE Anti-CD20 antibody [2H7]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD20 antibody [2H7]

  • 618 PE/DyLight™ 594

    PE/DyLight™ 594 Anti-CD20 antibody [2H7]

  • 675 PerCP

    PerCP Anti-CD20 antibody [2H7]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD20 antibody [2H7]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD20 antibody [2H7]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2H7

Isotype

IgG2b

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

No

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11836

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p>" } } }
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Product details

Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta naturae 12:51-55 PubMed32477598

2020

Establishment of a FDC-P1 Murine Cell Line with Human KIT N822K Gene Overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

E R Vagapova,T D Lebedev,V I Popenko,O G Leonova,P V Spirin,V S Prassolov
View all publications
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Product promise

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