AB157318

PerCP/Cy5.5® Anti-CD28 antibody [CD28.2]

Name: Anti-CD28 PerCP/Cy5.5® antibody [CD28.2] (ab157318) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB157318
Price: 250 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD28 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD28 protein.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, TP44

2 Images

Flow Cytometry - PerCP/Cy5.5® Anti-CD28 antibody [CD28.2] (AB157318)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD28 antibody [CD28.2] (AB157318)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab157318 (concentration in sample 1 μg/ml).

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD28 antibody [CD28.2] (AB157318)

Separation of human CD28 positive lymphocytes (red-filled) from CD28 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab157318 (concentration in sample 1 μg/ml).

Carrier free

Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CD28.2

Isotype

IgG1

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.

P10747

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. <a href='/en-us/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. <a href='/en-us/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Product details

Purified antibody is conjugated with tandem dye PerCP/Cy5.5^® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.

Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).

See full target information CD28

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Biochemical journal 293 ( Pt 3):835-42 PubMed8394695

1993

Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity.

Applications

Unspecified application

Species

Unspecified reactive species

J Nunes,S Klasen,M D Franco,C Lipcey,C Mawas,M Bagnasco,D Olive

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP/Cy5.5® Anti-CD28 antibody [CD28.2]

Flow Cytometry - PerCP/Cy5.5® Anti-CD28 antibody [CD28.2] (AB157318)

Flow Cytometry - PerCP/Cy5.5® Anti-CD28 antibody [CD28.2] (AB157318)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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