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AB157317

PerCP/Cy5.5® Anti-CD3 antibody [UCHT1]

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(5 Publications)

Mouse Monoclonal CD3d antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - PerCP/Cy5.5® Anti-CD3 antibody [UCHT1] (AB157317)
  • Flow Cyt

Lab

Flow Cytometry - PerCP/Cy5.5® Anti-CD3 antibody [UCHT1] (AB157317)

Analysis by flow cytometry of ab157317 staining CD3 in human peripheral blood cells.

  • 660 APC

    APC Anti-CD3 antibody [UCHT1]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD3 antibody [UCHT1]

  • Biotin

    Biotin Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 antibody [UCHT1]

  • 578 PE

    PE Anti-CD3 epsilon antibody [UCHT1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD3 antibody [UCHT1]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD3 antibody [UCHT1]

  • 675 PerCP

    PerCP Anti-CD3 antibody [UCHT1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD3e antibody [UCHT1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD3e antibody [UCHT1]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [UCHT1]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD3 antibody [UCHT1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHT1

Isotype

IgG1

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

No

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

Specificity

ab157317 reacts with the epsilon chain of the CD3 complex.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/en-us/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/en-us/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 40:101831 PubMed38039947

2023

Silencing of PD-1 combined with EBV-specific killer T cells for the treatment of EBV-associated B lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaping Wang,Zhijuan Xu,Yanli Lai,Yanli Zhang,Ping Zhang,Qitian Mu,Shujun Yang,Yongcheng Sun,Lixia Sheng,Guifang Ouyang

The Journal of experimental medicine 204:2537-44 PubMed17923503

2007

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Gabrielle M Siegers,Mahima Swamy,Edgar Fernández-Malavé,Susana Minguet,Sylvia Rathmann,Alberto C Guardo,Verónica Pérez-Flores,Jose R Regueiro,Balbino Alarcón,Paul Fisch,Wolfgang W A Schamel

The New England journal of medicine 354:1913-21 PubMed16672702

2006

Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric Rieux-Laucat,Claire Hivroz,Annick Lim,Véronique Mateo,Isabelle Pellier,Françoise Selz,Alain Fischer,Françoise Le Deist

Proceedings of the National Academy of Sciences of 101:16268-73 PubMed15534202

2004

Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly L Arnett,Stephen C Harrison,Don C Wiley

Journal of immunology (Baltimore, Md. : 1950) 161:1113-22 PubMed9686569

1998

Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

S le Gouvello,V Manceau,A Sobel
View all publications
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